Western Blot (WB)
(OGA/O-GlcNAcase antibody (pAb) tested by Immunoprecipitation. 10 ul of OGA/O-GlcNAcase antibody was used to immunoprecipitate OGA/O-GlcNAcase from 400 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the OGA/O-GlcNAcase antibody at a dilution of 1:1,000.)
Rabbit anti-Human OGA/O-GlcNAcase Polyclonal Antibody | anti-OGA/O-GlcNAcase antibody
OGA/O-GlcNAcase antibody (pAb)
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Affinity Purified
Synonyms
OGA/O-GlcNAcase; Polyclonal Antibody; OGA/O-GlcNAcase antibody (pAb); MGEA5; OGA; MEA5; NCOAT; anti-OGA/O-GlcNAcase antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-OGA/O-GlcNAcase antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
IP: 10ul per IP
WB: 1:500-1:2,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
IP: 10ul per IP
WB: 1:500-1:2,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
Immunogen
This antibody was raised against a peptide within the N-terminal region of human OGA/ O-GlcNAcase.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(OGA/O-GlcNAcase antibody (pAb) tested by Immunoprecipitation. 10 ul of OGA/O-GlcNAcase antibody was used to immunoprecipitate OGA/O-GlcNAcase from 400 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the OGA/O-GlcNAcase antibody at a dilution of 1:1,000.)
Western Blot (WB)
(OGA/O-GlcNAcase antibody (pAb) tested by Immunoprecipitation. 10 ul of OGA/O-GlcNAcase antibody was used to immunoprecipitate OGA/O-GlcNAcase from 400 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the OGA/O-GlcNAcase antibody at a dilution of 1:1,000.)
Western Blot (WB)
(OGA/O-GlcNAcase pAb tested by Western blot. OGA/O-GlcNAcase detection by Western blot. The analysis was performed using 20 ug HeLa whole cell extract and OGA/O-GlcNAcase at a 1:1,000 dilution.)
Western Blot (WB)
(OGA/O-GlcNAcase pAb tested by Western blot. OGA/O-GlcNAcase detection by Western blot. The analysis was performed using 20 ug HeLa whole cell extract and OGA/O-GlcNAcase at a 1:1,000 dilution.)
Related Product Information for anti-OGA/O-GlcNAcase antibody
Short Description: Our OGA/O-GlcNAcase antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: OGA/O GlcNAcase (O-linked N-acetylglucosamine (GlcNAc) -ase) is a glycosidase that removes O-linked N-acetylglucosamine (O-GlcNAc) modifications from serine and threonine residues on nuclear and cytoplasmic proteins. This dynamic modification is catalyzed by OGT and plays a role in homeostatic mechanisms and influences gene expression.
Background: OGA/O GlcNAcase (O-linked N-acetylglucosamine (GlcNAc) -ase) is a glycosidase that removes O-linked N-acetylglucosamine (O-GlcNAc) modifications from serine and threonine residues on nuclear and cytoplasmic proteins. This dynamic modification is catalyzed by OGT and plays a role in homeostatic mechanisms and influences gene expression.
Product Categories/Family for anti-OGA/O-GlcNAcase antibody
NCBI and Uniprot Product Information
Molecular Weight
115kDa
Similar Products
Product Notes
The OGA/O-GlcNAcase (Catalog #AAA388891) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The OGA/O-GlcNAcase antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OGA/O-GlcNAcase can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Applications Validated: IP: 10ul per IP WB: 1:500-1:2,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Researchers should empirically determine the suitability of the OGA/O-GlcNAcase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OGA/O-GlcNAcase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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