Western Blot (WB)
(SSRP1 antibody (pAb) tested by Western blot. Nuclear extract of K-562 cells (30 ug) probed with SSRP1 antibody (1:500).)
Rabbit anti-Human SSRP1 Polyclonal Antibody | anti-SSRP1 antibody
SSRP1 antibody (pAb)
Gene Names
SSRP1; FACT; T160; FACT80
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
SSRP1; Polyclonal Antibody; SSRP1 antibody (pAb); FACT; T160; FACT80; anti-SSRP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-SSRP1 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This antibody was raised against a peptide within the N-terminal region of human SSRP1.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(SSRP1 antibody (pAb) tested by Western blot. Nuclear extract of K-562 cells (30 ug) probed with SSRP1 antibody (1:500).)
Western Blot (WB)
(SSRP1 antibody (pAb) tested by Western blot. Nuclear extract of K-562 cells (30 ug) probed with SSRP1 antibody (1:500).)
Related Product Information for anti-SSRP1 antibody
Short Description: Our SSRP1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: SSRP1 (Structure-Specific Recognition Protein 1) is a component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. SSRP1 and Supt16H form a stable heterodimer and this complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. The HMG box DNA-binding domain mediates DNA-binding with both affinity and specificity for DNA damaged globally with cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
Background: SSRP1 (Structure-Specific Recognition Protein 1) is a component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. SSRP1 and Supt16H form a stable heterodimer and this complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. The HMG box DNA-binding domain mediates DNA-binding with both affinity and specificity for DNA damaged globally with cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
Product Categories/Family for anti-SSRP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
81,075 Da
NCBI Official Full Name
FACT complex subunit SSRP1
NCBI Official Synonym Full Names
structure specific recognition protein 1
NCBI Official Symbol
SSRP1
NCBI Official Synonym Symbols
FACT; T160; FACT80
NCBI Protein Information
FACT complex subunit SSRP1; FACT 80 kDa subunit; FACTp80; chromatin-specific transcription elongation factor 80 kDa subunit; cisplatin-DNA SSRP; facilitates chromatin remodeling 80 kDa subunit; facilitates chromatin transcription complex 80 kDa subunit; f
UniProt Protein Name
FACT complex subunit SSRP1
UniProt Gene Name
SSRP1
UniProt Synonym Gene Names
FACT80; FACT 80 kDa subunit; FACTp80; hSSRP1
UniProt Entry Name
SSRP1_HUMAN
Uniprot Description
SSRP1: a subunit of the chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63.
Protein type: Transcription initiation complex; Transcription, coactivator/corepressor; RNA-binding; Nucleolus
Chromosomal Location of Human Ortholog: 11q12
Cellular Component: nucleoplasm; cytoplasm; nucleolus; chromosome; nucleus
Molecular Function: protein binding; DNA binding; chromatin binding
Biological Process: transcription from RNA polymerase II promoter; chromatin remodeling; regulation of transcription, DNA-dependent; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression; DNA repair; DNA replication
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Product Notes
The SSRP1 ssrp1 (Catalog #AAA388904) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SSRP1 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SSRP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:2,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the SSRP1 ssrp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SSRP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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