Testing Data
(Western blot analysis of extracts of variouscell lines, using HNF4A antibody.)
Rabbit HNF4A Antibody | anti-HNF4A antibody
HNF4A Antibody
Gene Names
HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
HNF4A; HNF4A Antibody; HNF4; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF14; anti-HNF4A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
474
Applicable Applications for anti-HNF4A antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthetic peptide of human HNF4A
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20°C or-80°C. Avoid freeze/thaw cycles.
Testing Data
(Western blot analysis of extracts of variouscell lines, using HNF4A antibody.)
Testing Data
(Western blot analysis of extracts of variouscell lines, using HNF4A antibody.)
Related Product Information for anti-HNF4A antibody
Hepatocyte nuclear factor 4alpha (HNF4alpha) is a transcription factor that belongs to the steroid hormone receptor superfamily and is enriched in liver (1). HNF4alpha, in association with PGC-1alpha, activates gluconeogenic genes such as phosphoenolpyruvate carboxykinase and glucose-6-phosphatase genes in fasted livers (2, 3). Conditional knockout of the HNF4alpha gene in the mouse liver destroys lipid homeostasis and leads to lipid accumulation in the liver and a reduction of serum cholesterol and triglyceride levels (4). Mutations in HNF4alpha have been linked to maturity-onset diabetes of the young (MODY) (5).
Product Categories/Family for anti-HNF4A antibody
References
1. Sladek, F.M. et al. (1990) Genes Dev. 4, 2353-2365. 2. Yoon, J.C. et al. (2001) Nature 413, 131-138. 3. Rhee, J. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 4012-4017. 4. Hayhurst, G.P. et al. (2001) Mol. Cell Biol. 21, 1393-1403. 5. Ellard, S. and Colclough, K. (2006) Hum. Mutat. 27, 854-869.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52kDa
NCBI Official Full Name
hepatocyte nuclear factor 4-alpha 2
NCBI Official Synonym Full Names
hepatocyte nuclear factor 4 alpha
NCBI Official Symbol
HNF4A
NCBI Official Synonym Symbols
TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
NCBI Protein Information
hepatocyte nuclear factor 4-alpha
UniProt Protein Name
Hepatocyte nuclear factor 4-alpha
Protein Family
UniProt Gene Name
HNF4A
UniProt Synonym Gene Names
HNF4; NR2A1; TCF14; HNF-4-alpha; TCF-14
NCBI Description
The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]
Uniprot Description
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.
Research Articles on HNF4A
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Product Notes
The HNF4A hnf4a (Catalog #AAA8505949) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HNF4A Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HNF4A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the HNF4A hnf4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HNF4A, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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