Chromatin Immunoprecipitation (ChIP)
(Estrogen Receptor-a antibody (mAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of MCF7 cell chromatin (treated with 10nM estradiol for 1 hour) and 10 ug of Estrogen Receptor-a antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Mouse anti-Human Estrogen Receptor alpha Monoclonal Antibody | anti-ESR1 antibody
Estrogen Receptor alpha antibody (mAb)(Clone F3)
Gene Names
ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunoprecipitation, Western Blot
Purity
Protein G Chromatography
Synonyms
Estrogen Receptor alpha; Monoclonal Antibody; Estrogen Receptor alpha antibody (mAb)(Clone F3); ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1; anti-ESR1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
F3
Purity/Purification
Protein G Chromatography
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
0.61ug/ul (varies by lot)
Applicable Applications for anti-ESR1 antibody
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:1,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
WB: 1:500-1:1,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
Immunogen
This ER-alpha antibody was raised against a peptide within the C-terminal region of human Estrogen Receptor alpha.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(Estrogen Receptor-a antibody (mAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of MCF7 cell chromatin (treated with 10nM estradiol for 1 hour) and 10 ug of Estrogen Receptor-a antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Chromatin Immunoprecipitation (ChIP)
(Estrogen Receptor-a antibody (mAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of MCF7 cell chromatin (treated with 10nM estradiol for 1 hour) and 10 ug of Estrogen Receptor-a antibody. ChIP DNA was used in qPCR with the negative control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Related Product Information for anti-ESR1 antibody
Short Description: Our Estrogen Receptor alpha antibody (mAb)(Clone F3) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: ER-a (estrogen receptor a) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding and activation of transcription. ER-a localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer and osteoporosis.
Background: ER-a (estrogen receptor a) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding and activation of transcription. ER-a localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer and osteoporosis.
Product Categories/Family for anti-ESR1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
2,526 Da
NCBI Official Full Name
estrogen receptor isoform 1
NCBI Official Synonym Full Names
estrogen receptor 1
NCBI Official Symbol
ESR1
NCBI Official Synonym Symbols
ER; ESR; Era; ESRA; ESTRR; NR3A1
NCBI Protein Information
estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; nuclear receptor subfamily 3 group A member 1
UniProt Protein Name
Estrogen receptor
Protein Family
UniProt Entry Name
Q9UBT1_HUMAN
Similar Products
Product Notes
The ESR1 (Catalog #AAA388729) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Estrogen Receptor alpha antibody (mAb)(Clone F3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Estrogen Receptor alpha can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western Blot (WB). Applications Validated: WB: 1:500-1:1,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Researchers should empirically determine the suitability of the ESR1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Estrogen Receptor alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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