Rabbit Estrogen receptor Polyclonal Antibody | anti-ESR1 antibody

Anti-Estrogen Receptor Antibody

Cat. Num. AAA177419

• Gene Names: ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
• Reactivity: Reactis with: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Estrogen receptor; Polyclonal Antibody; Anti-Estrogen Receptor Antibody; anti-ESR1 antibody

• Host: Rabbit
• Reactivity: Reactis with: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other species
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 595

• Applicable Applications for anti-ESR1 antibody: Western Blot (WB), Immunohistochemistry (IHC-P)
• Application Notes: Western Blot: Concentration: 0.1-0.5mug/ml; Tested Species: Human; Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1mug/ml; Tested Species: Human; Antigen Retrieval: By Heat; ; WB: The detection limit for Estrogen Receptor is approximately 0.2ng/lane under reducing conditions.; Tested Species: In-house tested species with positive results.; By Heat Boiling: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formallin/paraffin sections. ; Other applications have not been tested.; Optimal dilutions should be detemined by end users.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Immunogen: E.coli-derived human Estrogen Receptor recombinant protein (Position: F425-V595). Human Estrogen Receptor shares 89% and 88% amino acid (aa) sequences identity with mouse and rat Estrogen Receptor, respectively.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- Estrogen Receptor antibody, MBS177419, Western blottingAll lanes: Anti Estrogen Receptor (MBS177419) at 0.5ug/mlWB: Recombinant Human ER Protein 0.5ngPredicted bind size: 37KDObserved bind size: 37KD )

Western Blot (WB)

(Anti- Estrogen Receptor antibody, MBS177419, Western blottingAll lanes: Anti Estrogen Receptor (MBS177419) at 0.5ug/mlWB: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 66KDObserved bind size: 66KD )

Immunohistochemistry (IHC)

(Anti- Estrogen Receptor antibody, MBS177419, IHC(P)IHC(P): Human Mammary Cancer Tissue)

Related Product Information for anti-ESR1 antibody

Description: Estrogen receptor alpha (ER-α), also known as NR3A1, is one of two main types of estrogen receptor, a nuclear receptor that is activated by the sex hormone estrogen. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. In humans, ER-α is encoded by the gene ESR1 (Estrogen Receptor 1). It is mapped to 6q25.1. This gene is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, it also play a role in other tissues such as bone.

NCBI and Uniprot Product Information

• NCBI GI #: 62821794
• NCBI GeneID: 2099
• NCBI Accession #: NP_000116.2
• NCBI GenBank Nucleotide #: NM_000125.3
• UniProt Accession #: P03372; Q13511; Q14276; Q5T5H7; Q6MZQ9; Q9NU51; Q9UDZ7; Q9UIS7
• NCBI Official Full Name: estrogen receptor isoform 1
• NCBI Official Synonym Full Names: estrogen receptor 1
• NCBI Official Symbol: ESR1
• NCBI Official Synonym Symbols: ER; ESR; Era; ESRA; ESTRR; NR3A1
• NCBI Protein Information: estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; nuclear receptor subfamily 3 group A member 1
• UniProt Protein Name: Estrogen receptor
• Protein Family: Estrogen receptor
• UniProt Gene Name: ESR1
• UniProt Synonym Gene Names: ESR; NR3A1; ER
• UniProt Entry Name: ESR1_HUMAN

NCBI Description

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Uniprot Description

ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; integral to membrane; plasma membrane; nucleus

Molecular Function: identical protein binding; protein binding; enzyme binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; estrogen response element binding; steroid hormone receptor activity; chromatin binding; transcription factor binding; transcription factor activity; steroid binding

Biological Process: positive regulation of nitric oxide biosynthetic process; estrogen receptor signaling pathway; signal transduction; uterus development; response to estradiol stimulus; regulation of apoptosis; positive regulation of fibroblast proliferation; elevation of cytosolic calcium ion concentration; regulation of transcription, DNA-dependent; epithelial cell development; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; negative regulation of transcription factor activity; antral ovarian follicle growth; positive regulation of nitric-oxide synthase activity; male gonad development; androgen metabolic process; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; chromatin remodeling; response to estrogen stimulus; gene expression; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); steroid hormone mediated signaling

Disease: Migraine With Or Without Aura, Susceptibility To, 1; Breast Cancer; Myocardial Infarction, Susceptibility To; Estrogen Resistance