Western Blot (WB)
(Rsf1 pAb tested by Western blot. Schneider's Drosophila L2 nuclear extract (10 ug) was probed with Rsf1 pAb (1:1,000 dilution).)
Rabbit anti-Drosophila Rsf1 Polyclonal Antibody | anti-Rsf1 antibody
Rsf1 antibody (pAb)
Reactivity
Drosophila
Applications
Western Blot
Purity
Affinity Purified
Synonyms
Rsf1; Polyclonal Antibody; Rsf1 antibody (pAb); CG8677; anti-Rsf1 antibody
Host
Rabbit
Reactivity
Drosophila
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.1% sodium azide, 0.2mg/ml BSA and 35% glycerol. Sodium azide is highly toxic.
Applicable Applications for anti-Rsf1 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:1,000-1:5,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
WB: 1:1,000-1:5,000 dilution
The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
Immunogen
This Rsf1 antibody was raised against a His-Tagged fusion protein corresponding to amino acids 165-350 of Drosophila Rsf1.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(Rsf1 pAb tested by Western blot. Schneider's Drosophila L2 nuclear extract (10 ug) was probed with Rsf1 pAb (1:1,000 dilution).)
Western Blot (WB)
(Rsf1 pAb tested by Western blot. Schneider's Drosophila L2 nuclear extract (10 ug) was probed with Rsf1 pAb (1:1,000 dilution).)
Related Product Information for anti-Rsf1 antibody
Short Description: Our Rsf1 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Drosophila samples.
Background: Rsf-1 (Remodeling and spacing factor 1) is a member of RSF complex. This complex consists of two subunits: Rsf-1 and SNF2H. RSF possesses a chromatin remodeling activity and a chromatin assembly activity. Rsf-1 assembles the nucleosomes randomly with its histone chaperone activity. The resulting nucleosomes are then redistributed by the ATP-utilizing nucleosome mobilization factor SNF2H into a periodic array. Rsf-1 can be involved in transcriptional repression when it is recruited to DNA via the DNA binding of Gal4 or in transcriptional coactivation when associated with hepatitis B virus pX protein (HBX). In response to hepatitis B infection, Rsf-1 can bind to the viral transcription activator pX, which then coactivates the transcription factor NFkB, thus supporting the life cycle of the viral genome. Additionally, Rsf-1 is thought to be a potent oncogene, with elevated expression observed in ovarian carcinomas.
Background: Rsf-1 (Remodeling and spacing factor 1) is a member of RSF complex. This complex consists of two subunits: Rsf-1 and SNF2H. RSF possesses a chromatin remodeling activity and a chromatin assembly activity. Rsf-1 assembles the nucleosomes randomly with its histone chaperone activity. The resulting nucleosomes are then redistributed by the ATP-utilizing nucleosome mobilization factor SNF2H into a periodic array. Rsf-1 can be involved in transcriptional repression when it is recruited to DNA via the DNA binding of Gal4 or in transcriptional coactivation when associated with hepatitis B virus pX protein (HBX). In response to hepatitis B infection, Rsf-1 can bind to the viral transcription activator pX, which then coactivates the transcription factor NFkB, thus supporting the life cycle of the viral genome. Additionally, Rsf-1 is thought to be a potent oncogene, with elevated expression observed in ovarian carcinomas.
Product Categories/Family for anti-Rsf1 antibody
NCBI and Uniprot Product Information
Molecular Weight
58kDa
Protein Family
Similar Products
Product Notes
The Rsf1 (Catalog #AAA388498) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rsf1 antibody (pAb) reacts with Drosophila and may cross-react with other species as described in the data sheet. AAA Biotech's Rsf1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:1,000-1:5,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Researchers should empirically determine the suitability of the Rsf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rsf1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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