Chromatin Immunoprecipitation (ChIP)
(Suz12 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from a human B cell lymphoma cell line and 10 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify Suz12 binding sites. The image shows binding across a region of chromosome 12.)
Rabbit anti-Human, Mouse Suz12 Polyclonal Antibody | anti-Suz12 antibody
Suz12 antibody (pAb)
ChIP: 10ul per ChIP
ChIP-Seq: 10ul each
WB: 1:250-1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. An additional band at approximately 20 kDa is also detected by this antibody in HeLa and HEK lysates, but not in mouse CSML0 cell lysate. For Suz12, we also offer AbFlex Suz12 Recombinant Antibody (rAb). For details
Chromatin Immunoprecipitation (ChIP)
(Suz12 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from a human B cell lymphoma cell line and 10 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify Suz12 binding sites. The image shows binding across a region of chromosome 12.)
Chromatin Immunoprecipitation (ChIP)
(Suz12 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from a human B cell lymphoma cell line and 10 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify Suz12 binding sites. The image shows binding across a region of chromosome 12.)
Chromatin Immunoprecipitation (ChIP)
(Suz12 antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human non-small cell lung carcinoma cell line NCI-H1299 cells and 10 ul of Suz12 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Chromatin Immunoprecipitation (ChIP)
(Suz12 antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human non-small cell lung carcinoma cell line NCI-H1299 cells and 10 ul of Suz12 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Western Blot (WB)
(Suz12 antibody (pAb) tested by Western blot. HeLa cell nuclear extract (20 ?g) was probed with Suz12 pAb (1:500 dilution).)
Western Blot (WB)
(Suz12 antibody (pAb) tested by Western blot. HeLa cell nuclear extract (20 ?g) was probed with Suz12 pAb (1:500 dilution).)
Background: Suz12 (Suppressor-of-Zeste 12 protein, also designated as ChET9, JJAZ1 protein) is a component of the Polycomb group complexes 2, 3 and 4 (PRC2, PRC3 and PRC4), which also contain the histone methyltransferase EZH2, EED (Embryonic Ectoderm Development protein) protein and the histone-binding proteins RbAP46 and RbAP48. PRC2 is responsible for di- and trimethylation of histone 3 at lysine 27 (Histone H3K27me2 and Histone H3K27me3), which leads to transcriptional repression of the target genes. Suz12 is a nuclear protein belonging to the VEFS family. Suz12 contains a zinc-finger binding motif and a VEFS box, which is required for the interaction between Suz12 and EZH2.
NCBI and Uniprot Product Information
Observed: 96 kDa
NCBI Description
This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. [provided by RefSeq, Jul 2009]
Uniprot Description
SUZ12: a zinc finger protein. A breakpoint translocation of the cognate gene with JAZF1 has been reported in endometrial stromal sarcoma.
Protein type: Oncoprotein; C2H2-type zinc finger protein; Transcription factor
Chromosomal Location of Human Ortholog: 17q11.2
Cellular Component: nucleoplasm; sex chromatin; ESC/E(Z) complex; nucleolus; nucleus
Molecular Function: histone methyltransferase activity; protein binding; sequence-specific DNA binding; RNA binding; metal ion binding; chromatin binding; methylated histone residue binding
Biological Process: histone methylation; negative regulation of cell differentiation; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; histone ubiquitination; positive regulation of cell proliferation; gene expression; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic
Research Articles on Suz12
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Product Notes
The Suz12 suz12 (Catalog #AAA388343) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Suz12 antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Suz12 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Immunofluorescence (IF), Western Blot (WB). Applications Validated: ChIP: 10ul per ChIP ChIP-Seq: 10ul each WB: 1:250-1:1,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. An additional band at approximately 20 kDa is also detected by this antibody in HeLa and HEK lysates, but not in mouse CSML0 cell lysate. For Suz12, we also offer AbFlex Suz12 Recombinant Antibody (rAb). For details. Researchers should empirically determine the suitability of the Suz12 suz12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Suz12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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