SDS-Page
(Recombinant NSD3 (WHSC1L1)-SET protein gel. Recombinant NSD3 (WHSC1L1)-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 60kDa Purity: > 90%)
NSD3 recombinant protein
Recombinant NSD3 (WHSC1L1)-SET protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant NSD3 (WHSC1L1)-SET protein gel. Recombinant NSD3 (WHSC1L1)-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 60kDa Purity: > 90%)
SDS-Page
(Recombinant NSD3 (WHSC1L1)-SET protein gel. Recombinant NSD3 (WHSC1L1)-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 60kDa Purity: > 90%)
Western Blot (WB)
(Recombinant NSD3 (WHSC1L1)-SET activity assay. 2 ug recombinant nucleosomes were incubated with NSD3 (WHSC1L1)-SET protein in 30 ul reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 h at RT. 5 ul reaction products were loaded on a 12.5% SDS-PAGE gel. Western Blot was used to detect reaction products (H3K36me2). NSD3 (WHSC1L1)-SET only was used as negative control. Results show that the activity of NSD3 (WHSC1L1)-SET with nucleosomes as substrate is much better than its activity with histone octamers as substrate.)
Western Blot (WB)
(Recombinant NSD3 (WHSC1L1)-SET activity assay. 2 ug recombinant nucleosomes were incubated with NSD3 (WHSC1L1)-SET protein in 30 ul reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 h at RT. 5 ul reaction products were loaded on a 12.5% SDS-PAGE gel. Western Blot was used to detect reaction products (H3K36me2). NSD3 (WHSC1L1)-SET only was used as negative control. Results show that the activity of NSD3 (WHSC1L1)-SET with nucleosomes as substrate is much better than its activity with histone octamers as substrate.)
Background: NSD3 (WHSC1L1/Wolf-Hirschhorn Syndrome Candidtae 1-Like 1) is a histone methyltransferase of NSD (nuclear receptor binding SET domain protein) family. NSD3 (WHSC1L1) can recognize and methylate several lysine marks if using histone as substrate. However, if using nucleosome as substrate, NSD3 (WHSC1L1) preferentially methylates lysine 36 of histone H3. The NSD3 (WHSC1L1) gene is amplified in several cancers, including lung cancer and head and neck cancer, and may play a role in carcinogenesis. NSD3 (WHSC1L1) includes the SET domain of NSD3 (WHSC1L1). The SET domain is a 130 to 140 amino acid, evolutionary conserved motif that was initially characterized in Drosophila proteins Su(var)3-9, EZH2, and TrxG, and plays a crucial role in substrate recognition and enzymatic activity.
NCBI and Uniprot Product Information
NCBI Description
This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. [provided by RefSeq, May 2015]
Uniprot Description
WHSC1L1: is related to the Wolf-Hirschhorn syndrome candidate-1 protein containing PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced isoforms have been described.
Protein type: Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine; Oncoprotein; Histone-binding; EC 2.1.1.43; Methyltransferase
Chromosomal Location of Human Ortholog: 8p11.2
Cellular Component: nucleoplasm; chromosome; nucleus
Molecular Function: protein binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; histone lysine N-methyltransferase activity (H3-K27 specific)
Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; histone H3-K4 methylation; negative regulation of transcription, DNA-dependent
Disease: Leukemia, Acute Myeloid
Research Articles on NSD3
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Product Notes
The NSD3 whsc1l1 (Catalog #AAA388188) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NSD3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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