Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSD3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HeLa cell lysate Lane 3.: MCF7 cell lysate Lane 4.: NIH3T3 cell lysate Lane 5.: U87MG cell lysate)
Mouse anti-Human NSD3 Monoclonal Antibody | anti-NSD3 antibody
NSD3 antibody
Gene Names
NSD3; KMT3F; KMT3G; WHISTLE; WHSC1L1; pp14328
Reactivity
Human
Applications
ELISA, Western Blot
Purity
By protein-G affinity chromatography
Synonyms
NSD3; Monoclonal Antibody; NSD3 antibody; Transcription elongation factor B polypeptide 3 binding protein 1; DKFZp667H044; FLJ20353; Histone lysine N methyltransferase NSD3; MGC126766; MGC142029; NSD 3; Nuclear set domain containing 3; Nuclear SET domain containing protein 3; pp14328; Whistle; WHSC1 like protein 1; WHSC1L1 protein; Wolf Hirschhorn syndrome candidate 1 like 1; ; anti-NSD3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT7B4
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1437
Applicable Applications for anti-NSD3 antibody
ELISA (EIA), Western Blot (WB)
Antigen Species
Human
Immunogen
Recombinant human NSD3 (383-660aa) purified from E. coli
Clone Note
Anti-human NSD3 mAb, clone AT7B4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NSD3 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSD3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HeLa cell lysate Lane 3.: MCF7 cell lysate Lane 4.: NIH3T3 cell lysate Lane 5.: U87MG cell lysate)
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NSD3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HeLa cell lysate Lane 3.: MCF7 cell lysate Lane 4.: NIH3T3 cell lysate Lane 5.: U87MG cell lysate)
Related Product Information for anti-NSD3 antibody
This gene encoding NSD3 is related to the Wolf-Hirschhorn syndrome candidate - 1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. NSD3 is Histone methyltransferase which preferentially methylates 'Lys-4' and 'Lys-27' of histone H3 and is amplified in several tumor cell lines and primary breast carcinomas.
Product Categories/Family for anti-NSD3 antibody
References
Angrand P.O, et al. (2001) Genomics 74 (1):79-88; Stec I, et al. (2000) FEBS Lett 473 (1):1-5
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
160,314 Da
NCBI Official Full Name
histone-lysine N-methyltransferase NSD3 isoform long
NCBI Official Synonym Full Names
nuclear receptor binding SET domain protein 3
NCBI Official Symbol
NSD3
NCBI Official Synonym Symbols
KMT3F; KMT3G; WHISTLE; WHSC1L1; pp14328
NCBI Protein Information
histone-lysine N-methyltransferase NSD3
UniProt Protein Name
Histone-lysine N-methyltransferase NSD3
UniProt Gene Name
NSD3
UniProt Synonym Gene Names
WHSC1-like protein 1
NCBI Description
This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. [provided by RefSeq, May 2015]
Uniprot Description
Histone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.
Research Articles on NSD3
Similar Products
Product Notes
The NSD3 nsd3 (Catalog #AAA200067) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NSD3 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NSD3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the NSD3 nsd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NSD3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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