ELISA (EIA)
(TransAM standard curve generated using Recombinant c-Fos protein. The standard curve for TransAM was generated using a range of 40-10 ng of protein and run on the TransAM AP-1 c-Fos ELISA Kit.)
c-Fos recombinant protein
Recombinant c-Fos protein
Purity
The purified recombinant protein is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity.
Synonyms
c-Fos; Recombinant c-Fos protein; p55; AP-1; FOS; c-Fos recombinant protein
Host
Baculovirus
Purity/Purification
The purified recombinant protein is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity.
Form/Format
5ug of Recombinant c-Fos protein and Dilution Buffer AM1 (20mM Tris-Cl (pH8), 20% glycerol, 100mM KCl, 1mM DTT and 0.2mM EDTA). Protein is supplied at 0.5ug/ul in Dilution Buffer AM1.concentration.
Protein Species
Human
Tag
His-Tag
Notes
Recombinant c-Fos is suitable for DNA and protein-protein interaction assays. 100ng is sufficient for DNA-protein and protein-protein interaction studies. The standard curve for TransAM AP-1 c-Fos was generated using the range of 40-2.5ng of protein. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
ELISA (EIA)
(TransAM standard curve generated using Recombinant c-Fos protein. The standard curve for TransAM was generated using a range of 40-10 ng of protein and run on the TransAM AP-1 c-Fos ELISA Kit.)
ELISA (EIA)
(TransAM standard curve generated using Recombinant c-Fos protein. The standard curve for TransAM was generated using a range of 40-10 ng of protein and run on the TransAM AP-1 c-Fos ELISA Kit.)
Related Product Information for c-Fos recombinant protein
Short Description: Recombinant c-Fos was expressed from full-length (accession number NM 003131) with an N-terminal His-Tag in a baculovirus system and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity. c-Fos is one of the proteins that form the heteromeric AP-1 transcription factor complex. AP-1 proteins play a role in the expression of many genes involved in the regulation of cellular processes such as differentiation, proliferation and apoptosis. The transcription factor AP-1 is composed of a mixture of heterodimeric protein complexes derived from the Fos and Jun families, including c-Fos, FosB, Fra-1, c-Jun, JunB and JunD. c-Fos is a nuclear phosphoprotein that forms a tight but non-covalently linked complex with the Jun/AP-1 transcription factor. In the heterodimer, Fos and Jun/AP-1 basic regions each seem to interact with symmetrical DNA half sites. Upon TGF-b activation, c-Fos forms a multimeric SMAD3/SMAD4/Jun/Fos complex at the AP-1/SMAD-binding site to regulate TGF-b-mediated signaling. c-Fos has a critical function in regulating bone morphogenesis. Recombinant c-Fos is suitable for DNA-protein (gelshift) and protein-protein interaction assays.
Background: c-Fos is one of the proteins that form the heteromeric AP-1 transcription factor complex. AP-1 proteins play a role in the expression of many genes involved in the regulation of cellular processes such as differentiation, proliferation and apoptosis. The transcription factor AP-1 is composed of a mixture of heterodimeric protein complexes derived from the Fos and Jun families, including c-Fos, FosB, Fra-1, c-Jun, JunB and JunD. c-Fos is a nuclear phosphoprotein that forms a tight but non-covalently linked complex with the Jun/AP-1 transcription factor. In the heterodimer, Fos and Jun/AP-1 basic regions each seem to interact with symmetrical DNA half sites. Upon TGF-b activation, c-Fos forms a multimeric SMAD3/SMAD4/Jun/Fos complex at the AP-1/SMAD-binding site to regulate TGF-b-mediated signaling. c-Fos has a critical function in regulating bone morphogenesis.
Background: c-Fos is one of the proteins that form the heteromeric AP-1 transcription factor complex. AP-1 proteins play a role in the expression of many genes involved in the regulation of cellular processes such as differentiation, proliferation and apoptosis. The transcription factor AP-1 is composed of a mixture of heterodimeric protein complexes derived from the Fos and Jun families, including c-Fos, FosB, Fra-1, c-Jun, JunB and JunD. c-Fos is a nuclear phosphoprotein that forms a tight but non-covalently linked complex with the Jun/AP-1 transcription factor. In the heterodimer, Fos and Jun/AP-1 basic regions each seem to interact with symmetrical DNA half sites. Upon TGF-b activation, c-Fos forms a multimeric SMAD3/SMAD4/Jun/Fos complex at the AP-1/SMAD-binding site to regulate TGF-b-mediated signaling. c-Fos has a critical function in regulating bone morphogenesis.
Product Categories/Family for c-Fos recombinant protein
NCBI and Uniprot Product Information
Similar Products
Product Notes
The c-Fos (Catalog #AAA388008) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "c-Fos, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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