ELISA (EIA)
(TransAM standard curve generated using Recombinant p53 protein. The standard curve for TransAM was generated using a range of 40 – 0.625 ng of protein and run on the TransAM p53 ELISA Kit.)
p53 recombinant protein
Recombinant p53 protein
Synonyms
p53; Recombinant p53 protein; TP53; BCC7; LFS1; TRP53; p53 recombinant protein
Host
Baculovirus
Form/Format
5ug of Recombinant p53 protein and 1X Protein Dilution Buffer (20mM Tris-HCl pH8.0, 20% glycerol, 100mM KCl, 0.2mM EDTA and 1mM DTT. Protein is supplied at 0.5ug/ul in Protein Dilution Buffer.
Protein Species
Human
Tag
His-Tag
Notes
Recombinant p53 is suitable for TransAM, protein-protein and DNA protein assays. 1ng is sufficient for a gelshift assay. The standard curve for TransAM p53 was generated using the range of 40-0.625ng of protein. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
ELISA (EIA)
(TransAM standard curve generated using Recombinant p53 protein. The standard curve for TransAM was generated using a range of 40 – 0.625 ng of protein and run on the TransAM p53 ELISA Kit.)
ELISA (EIA)
(TransAM standard curve generated using Recombinant p53 protein. The standard curve for TransAM was generated using a range of 40 – 0.625 ng of protein and run on the TransAM p53 ELISA Kit.)
Related Product Information for p53 recombinant protein
Short Description: Full-length recombinant p53 protein was expressed in a baculovirus system with a His-Tag and purified by an affinity column in combination with FPLC chromatography. We also offers Recombinant p53 protein that was expressed in E Coli. p53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, an ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFkB (NFkB p50 and >NFkB p65), both of which serve to inhibit apoptosis. Recombinant p53 is used in TransAM, DNA-protein (gelshift) and protein-protein interaction assays.
Background: p53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, an ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFkB (NFkB p50 and NFkB p65), both of which serve to inhibit apoptosis.
Background: p53 is the most important tumor suppressor in the genome. It is responsive to numerous genotoxic stresses, which activates its transcription factor activity, in turn causing cell-cycle arrest by activating expression of p21 Cip/WAF. Mutant p53 that has lost its DNA-binding function interferes with the activity of native p53 and leads to oncogenic transformation. Alternatively, transformation may be caused by overexpression of Mdm2/Hdm2, an ubiquitin ligase specific for p53, which causes its destabilization. Inactivation of p53 is often coincident with hyperactivation of NFkB (NFkB p50 and NFkB p65), both of which serve to inhibit apoptosis.
Product Categories/Family for p53 recombinant protein
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Product Notes
The p53 (Catalog #AAA388006) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "p53, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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