Western Blot (WB)
Mouse anti-Human, mouse Octamer-binding transcription factor 4, OCT-4 Polyclonal Antibody | anti-OCT-4 antibody
Anti-Octamer-binding transcription factor 4, OCT-4 antibody
Reactivity
Human, mouse
Applications
Western Blot, Immunocytochemistry
Purity
Protein A or Protein G purified
Synonyms
Octamer-binding transcription factor 4; OCT-4; Polyclonal Antibody; Anti-Octamer-binding transcription factor 4; OCT-4 antibody; Monoclonal Anti- Octamer-binding transcription factor 4; anti-OCT-4 antibody
Host
Mouse
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
IgG1
Clone Number
N65
Purity/Purification
Protein A or Protein G purified
Form/Format
Liquid
Applicable Applications for anti-OCT-4 antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
WB (1:1000); ICC (1:50)
Immunogen
Recombinant OCT-4 protein.
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
Preparation and Storage
Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-OCT-4 antibody
Oct-4 (octamer-binding transcription factor 4) also known as POU5F1 (POU domain, class 5, transcription factor 1) is a homeodomain transcription factor of the POU family. This protein is critically involved in the self-renewal of undifferentiated embryonic stem cells. [1] As such, it is frequently used as a marker for undifferentiated cells. A critical amount of Oct3/4 is required to sustain stem cell self-renewal, and up- or downregulation induces divergent developmental programs. [2] It has also been showed that Oct3 has a role in cardiac development in the early mouse embryo. [3] Oct-4 expression must be closely regulated; too much or too little will cause differentiation of the cells. [4]
References
1. Young Lab- Core Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells at MIT. 2. Niwa, H., Miyazaki, J., Smith, A. G. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nature Genet. 24: 372-376, 2000. 3. Zeineddine, D., Papadimou, E., Chebli, K., Gineste, M., Liu, J., Grey, C., Thurig, S., Behfar, A., Wallace, V. A., Skerjanc, I. S., Puceat, M. Oct-3/4 dose dependently regulates specification of embryonic stem cells toward a cardiac lineage and early heart development. Dev. Cell 11: 535-546, 2006. 4. Niwa H, Miyazaki J, Smith AG (April 2000). "Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells". Nat. Genet. 24 (4): 372-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
octamer-binding transcription factor 4, partial
Similar Products
Product Notes
The OCT-4 (Catalog #AAA355082) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Octamer-binding transcription factor 4, OCT-4 antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Octamer-binding transcription factor 4, OCT-4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). WB (1:1000); ICC (1:50). Researchers should empirically determine the suitability of the OCT-4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Octamer-binding transcription factor 4, OCT-4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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