IL-34 elisa kit

Human IL-34 ELISA kit

Cat. Num. AAA335766

• Gene Names: IL34; IL-34; C16orf77
• Reactivity: No cross reactivity observed with CD31, IL-10, IP-10, IL-31, IL-1a, IL-1b, GM-CSF, M-CSF, IL-29, MCP-1 and murine IL-34
• Synonyms: IL-34; Human IL-34 ELISA kit; IL-34 elisa kit

• Reactivity: No cross reactivity observed with CD31, IL-10, IP-10, IL-31, IL-1a, IL-1b, GM-CSF, M-CSF, IL-29, MCP-1 and murine IL-34
• Specificity: The assay recognizes natural and recombinant human IL-34. To define specificity of this ELISA, several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested (CD31, IL-10, IP-10, IL-31, IL-1IL-1GM-CSF, M-CSF, IL-29, MCP-1 and murine IL-34).
• Sequence Length: 242

• Samples: Cell culture supernatants, buffered solutions or human serum, plasma, or other body fluids. This assay will recognize both natural and recombinant human IL-34
• Assay Type: Sandwich
• Sensitivity: 39 pg/ml
• Intra-assay Precision: Reproducibility within the assay was evaluated in three independent experiments. Each assay was carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in culture media and 2 in standard diluent with samples containing different concentrations of IL-34. The overall intra-assay coefficient of variation has been calculated to be 5.3%.
• Inter-assay Precision: Assay to assay reproducibility within one laboratory was evaluated in three independent experiments. Each assay was carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in culture media and 2 in standard diluent with samples containing different concentrations of IL-34. The calculated overall coefficient of variation was 7.8%.

Related Product Information for IL-34 elisa kit

Intended Uses: The human IL-34 ELISA is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of IL-34 in cell culture supernatants, buffered solutions or human serum, plasma, or other body fluids. This assay will recognize both natural and recombinant human IL-34

Principle of the Assay: A capture Antibody highly specific for IL-34 has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of IL-34 in samples and known standards to the capture antibodies is completed and then any excess unbound analyte is removed. During the next incubation period the binding of the biotinylated anti-IL-34 secondary antibody to the analyte occurs. Any excess unbound secondary antibody is then removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of IL-34 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of IL-34 in any sample tested.

NCBI and Uniprot Product Information

• NCBI GI #: 289666752
• NCBI GeneID: 146433
• NCBI Accession #: NP_001166243.1
• NCBI GenBank Nucleotide #: NM_001172772.1
• Molecular Weight: 27,354 Da
• NCBI Official Full Name: interleukin-34 isoform 1
• NCBI Official Synonym Full Names: interleukin 34
• NCBI Official Symbol: IL34
• NCBI Official Synonym Symbols: IL-34; C16orf77
• NCBI Protein Information: interleukin-34
• UniProt Protein Name: Interleukin-34
• UniProt Gene Name: IL34
• UniProt Synonym Gene Names: C16orf77; IL-34
• UniProt Entry Name: IL34_HUMAN

NCBI Description

Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM, May 2008]

Uniprot Description

IL34: Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Belongs to the IL-34 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cytokine; Secreted; Cell cycle regulation

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: extracellular region; extracellular space

Molecular Function: cytokine activity; growth factor activity; macrophage colony stimulating factor receptor binding

Biological Process: inflammatory response; innate immune response; positive regulation of cell proliferation; positive regulation of macrophage differentiation; positive regulation of monocyte differentiation; positive regulation of protein amino acid phosphorylation