Immunofluorescence (IF)
(Sample Type :HA-MAPK15 transfected HeLa cellsPrimary Antibody Dilution :1:200Secondary Antibody :Anti-rabbit-Alexa Fluor 555Secondary Antibody Dilution :1:800Color/Signal Descriptions :A: Anti-HA-Alexa Fluor 488 B: MAPK15 C: DAPI D: MergeGene Name :MAPK15Submitted by :David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life Sciences)
Rabbit anti-Human, Rat MAPK15 Polyclonal Antibody | anti-MAPK15 antibody
MAPK15 antibody - middle region
Immunofluorescence (IF)
(Sample Type :HA-MAPK15 transfected HeLa cellsPrimary Antibody Dilution :1:200Secondary Antibody :Anti-rabbit-Alexa Fluor 555Secondary Antibody Dilution :1:800Color/Signal Descriptions :A: Anti-HA-Alexa Fluor 488 B: MAPK15 C: DAPI D: MergeGene Name :MAPK15Submitted by :David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life Sciences)
Immunofluorescence (IF)
(Sample Type :HA-MAPK15 transfected HeLa cellsPrimary Antibody Dilution :1:200Secondary Antibody :Anti-rabbit-Alexa Fluor 555Secondary Antibody Dilution :1:800Color/Signal Descriptions :A: Anti-HA-Alexa Fluor 488 B: MAPK15 C: DAPI D: MergeGene Name :MAPK15Submitted by :David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life Sciences)
Western Blot (WB)
(Lanes:Lane 1: 50ug untransfected HEK-293T lysateLane 2: 50ug MAPK15 transfected HEK-293T lysatePrimary Antibody Dilution:1:2000Secondary Antibody:Anti-rabbit-HRPSecondary Antibody Dilution:1:1000Gene Name:MAPK15Submitted by:David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life Sciences)
Western Blot (WB)
(Lanes:Lane 1: 50ug untransfected HEK-293T lysateLane 2: 50ug MAPK15 transfected HEK-293T lysatePrimary Antibody Dilution:1:2000Secondary Antibody:Anti-rabbit-HRPSecondary Antibody Dilution:1:1000Gene Name:MAPK15Submitted by:David Colecchia, Ph.D, Istituto Toscano Tumori, Core Research Laboratory, presso Fondazione Toscana Life Sciences)
Western Blot (WB)
(WB Suggested Anti-MAPK15 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate)
Western Blot (WB)
(WB Suggested Anti-MAPK15 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: MCF7 cell lysate)
Target Description: In vitro, MAPK15 phosphorylates MBP.
NCBI and Uniprot Product Information
Uniprot Description
ERK7: In vitro, phosphorylates MBP. Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Kinase, protein; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; MAPK family; Erk7 subfamily
Chromosomal Location of Human Ortholog: -
Cellular Component: extracellular region; intracellular; nucleus
Molecular Function: MAP kinase activity; SH3 domain binding; ATP binding
Biological Process: negative regulation of DNA replication; positive regulation of protein ubiquitination; protein amino acid autophosphorylation; positive regulation of protein catabolic process; MAPKKK cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; response to estradiol stimulus
Research Articles on MAPK15
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Product Notes
The MAPK15 mapk15 (Catalog #AAA3212219) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAPK15 antibody - middle region reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAPK15 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). Researchers should empirically determine the suitability of the MAPK15 mapk15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: GHDPAEHESP RAAKNVPRQN SAPLLQTALL GNGERPPGAK EAPPLTLSLV. It is sometimes possible for the material contained within the vial of "MAPK15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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