Immunohistochemistry (IHC)
(Sample Type :Untransfected HEK293 and Sema3F-AP transfected HEK293 Primary Antibody Dilution :1:1000 Secondary Antibody :Anti rabbit-Alexa Fluor 488 Secondary Antibody Dilution :1:500 Color/Signal Descriptions :Gene Name :SEMA3FSubmitted by :Dr. Tracy Tran, Rutgers University )
Rabbit Sema3f Polyclonal Antibody | anti-SEMA3F antibody
Sema3f antibody - N-terminal region
Immunohistochemistry (IHC)
(Sample Type :Untransfected HEK293 and Sema3F-AP transfected HEK293 Primary Antibody Dilution :1:1000 Secondary Antibody :Anti rabbit-Alexa Fluor 488 Secondary Antibody Dilution :1:500 Color/Signal Descriptions :Gene Name :SEMA3FSubmitted by :Dr. Tracy Tran, Rutgers University )
Immunohistochemistry (IHC)
(Sample Type :Untransfected HEK293 and Sema3F-AP transfected HEK293 Primary Antibody Dilution :1:1000 Secondary Antibody :Anti rabbit-Alexa Fluor 488 Secondary Antibody Dilution :1:500 Color/Signal Descriptions :Gene Name :SEMA3FSubmitted by :Dr. Tracy Tran, Rutgers University )
Immunoprecipitation (IP)
(Researcher: Dr. Chrisna LeVaillant, School of Anatomy and Human BiologyApplication: IPSpecies+tissue/cell type:1.5mL conditioned media from HEK293F cells transfected with 1: Sema 3F_AP, 2: Sema3C_DYKDDDDK, 3: UntransfectedAmount of IP Antibody: Amount of IP Antibody: 5ugPrimary antibody dilution: 1:1000Secondary antibody: Goat Anti-rabbit HRPSecondary antibody dilution:1:10000)
Immunoprecipitation (IP)
(Researcher: Dr. Chrisna LeVaillant, School of Anatomy and Human BiologyApplication: IPSpecies+tissue/cell type:1.5mL conditioned media from HEK293F cells transfected with 1: Sema 3F_AP, 2: Sema3C_DYKDDDDK, 3: UntransfectedAmount of IP Antibody: Amount of IP Antibody: 5ugPrimary antibody dilution: 1:1000Secondary antibody: Goat Anti-rabbit HRPSecondary antibody dilution:1:10000)
Western Blot (WB)
(WB Suggested Anti-SEMA3F AntibodyPositive Control: Lane1: No transfection, Lane, 2: Cell lysates from HEK293 cells were transfected with alkaline phosphatase (AP-) tagged Sema3F, Lane3: AP-alone, Lane4: The cell media from cells transfected with AP-Sema3FPrimary Antibody Dilution : 1ug/mlSecondary Antibody : Anti-APSecondry Antibody Dilution : 1:1000Submitted by: Dr. Tracy Tran, Rutgers University)
Western Blot (WB)
(WB Suggested Anti-SEMA3F AntibodyPositive Control: Lane1: No transfection, Lane, 2: Cell lysates from HEK293 cells were transfected with alkaline phosphatase (AP-) tagged Sema3F, Lane3: AP-alone, Lane4: The cell media from cells transfected with AP-Sema3FPrimary Antibody Dilution : 1ug/mlSecondary Antibody : Anti-APSecondry Antibody Dilution : 1:1000Submitted by: Dr. Tracy Tran, Rutgers University)
Western Blot (WB)
(WB Suggested Anti-Sema3f AntibodyTitration: 1.0 ug/mlPositive Control: Mouse Liver)
Western Blot (WB)
(WB Suggested Anti-Sema3f AntibodyTitration: 1.0 ug/mlPositive Control: Mouse Liver)
Target Description: The function remains unknown.
NCBI and Uniprot Product Information
Uniprot Description
SEMA3F: May play a role in cell motility and cell adhesion. Belongs to the semaphorin family.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted
Cellular Component: extracellular region; extracellular space
Molecular Function: chemorepellent activity; semaphorin receptor binding
Biological Process: axon extension involved in axon guidance; axon guidance; branchiomotor neuron axon guidance; facial nerve structural organization; negative chemotaxis; negative regulation of axon extension involved in axon guidance; nerve development; neural crest cell migration; positive regulation of cell migration; trigeminal nerve structural organization
Research Articles on SEMA3F
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Product Notes
The SEMA3F sema3f (Catalog #AAA3212858) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Sema3f antibody - N-terminal region reacts with Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's Sema3f can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the SEMA3F sema3f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: FIHAELIPDS AERNDDKLYF FFRERSAEAP QNPAVYARIG RICLNDDGGH. It is sometimes possible for the material contained within the vial of "Sema3f, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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