Immunohistochemistry (IHC)
(Sample Type :Human brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :UBE2N: Red DAPI:BlueGene Name :UBE2NSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science)
Rabbit UBE2N Polyclonal Antibody | anti-UBE2N antibody
UBE2N antibody - middle region
Immunohistochemistry (IHC)
(Sample Type :Human brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :UBE2N: Red DAPI:BlueGene Name :UBE2NSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science)
Immunohistochemistry (IHC)
(Sample Type :Human brain stem cellsPrimary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:1000Color/Signal Descriptions :UBE2N: Red DAPI:BlueGene Name :UBE2NSubmitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science)
Western Blot (WB)
(Host: MouseTarget Name: UBE2NSample Tissue: Mouse SpleenAntibody Dilution: 1ug/ml)
Western Blot (WB)
(Host: MouseTarget Name: UBE2NSample Tissue: Mouse SpleenAntibody Dilution: 1ug/ml)
Western Blot (WB)
(Lanes:1: 40ng HIS-UBE2D1 protein2: 40ng HIS-UBE2D2 protein3: 40ng HIS-UBE2D3 protein4: 40ng HIS-UBE2D4 protein5: 40ng HIS-UBE2E1 protein6: 40ng HIS-UBE2E2 protein7: 40ng HIS-UBE2E3 protein8: 40ng HIS-UBE2K protein9: 40ng HIS-UBE2L3 protein10: 40ng HIS-UBE2N protein11: 40ng HIS-UBE2V1 protein12: 40ng HIS-UBE2V2 protein.Primary Antibody Dilution:1:500Secondary Antibody:Anti-rabbit-HRPSecondary Antibody Dilution:1:50,000Gene Name:UBE2NSubmitted by:Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.)
Western Blot (WB)
(Lanes:1: 40ng HIS-UBE2D1 protein2: 40ng HIS-UBE2D2 protein3: 40ng HIS-UBE2D3 protein4: 40ng HIS-UBE2D4 protein5: 40ng HIS-UBE2E1 protein6: 40ng HIS-UBE2E2 protein7: 40ng HIS-UBE2E3 protein8: 40ng HIS-UBE2K protein9: 40ng HIS-UBE2L3 protein10: 40ng HIS-UBE2N protein11: 40ng HIS-UBE2V1 protein12: 40ng HIS-UBE2V2 protein.Primary Antibody Dilution:1:500Secondary Antibody:Anti-rabbit-HRPSecondary Antibody Dilution:1:50,000Gene Name:UBE2NSubmitted by:Dr Chris Boutell. MRC-UoG Centre for Virus Research (CVR), UK.)
Western Blot (WB)
(WB Suggested Anti-UBE2N Antibody Titration: 1.25ug/mlPositive Control: HepG2 cell lysateUBE2N is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells)
Western Blot (WB)
(WB Suggested Anti-UBE2N Antibody Titration: 1.25ug/mlPositive Control: HepG2 cell lysateUBE2N is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells)
Target Description: UBE2N encodes a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
NCBI and Uniprot Product Information
NCBI Description
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq, Jul 2008]
Uniprot Description
UBE2N: The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Heterodimer with UBE2V2. Interacts (UBE2V2-UBE2N heterodimer) with the E3 ligase STUB1 (via the U-box domain); the complex has a specific 'Lys-63'-linked polyubiquitination activity. Interacts with RNF8 and RNF168. Interacts with RNF11. Interacts with the E3 ligases, HLTF and SHPRH; the interactions promote the 'Lys-63'-linked polyubiquitination of PCNA upon genotoxic stress and lead to DNA repair. Interacts with ARIH2 (via RING-type 2). Belongs to the ubiquitin-conjugating enzyme family.
Protein type: Ligase; Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; DNA repair, damage
Chromosomal Location of Human Ortholog: 12q22
Cellular Component: protein complex; cytoplasm; nucleus; cytosol; ubiquitin ligase complex; UBC13-MMS2 complex
Molecular Function: ubiquitin binding; protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity
Biological Process: ubiquitin-dependent protein catabolic process; regulation of histone ubiquitination; positive regulation of DNA repair; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of histone modification; postreplication repair; cytokine and chemokine mediated signaling pathway; DNA double-strand break processing; protein modification process; protein ubiquitination; proteolysis; T cell receptor signaling pathway; double-strand break repair via homologous recombination; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of DNA repair; toll-like receptor 5 signaling pathway; positive regulation of ubiquitin-protein ligase activity; histone ubiquitination; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway
Research Articles on UBE2N
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Product Notes
The UBE2N ube2n (Catalog #AAA3201856) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE2N antibody - middle region reacts with Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Yeast, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2N can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the UBE2N ube2n for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: VDKLGRICLD ILKDKWSPAL QIRTVLLSIQ ALLSAPNPDD PLANDVAEQW. It is sometimes possible for the material contained within the vial of "UBE2N, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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