Mouse anti-Human AMACR/P504S Monoclonal Antibody | anti-AMACR antibody

AMACR/P504S Monoclonal Antibody

Cat. Num. AAA3014434

• Gene Names: AMACR; RM; RACE; CBAS4; P504S; AMACRD
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProG affinity purified.
• Synonyms: AMACR/P504S; Monoclonal Antibody; AMACR/P504S Monoclonal Antibody; AMACR; 2 arylpropionyl CoA epimerase; 2 methylacyl CoA racemase; 2-methylacyl-CoA racemase; Alpha methylacyl CoA racemase; Alpha methylacyl Coenzyme A racemase; Alpha methylacyl-CoA racemase deficiency; included; Alpha-methylacyl-CoA racemase; Amacr; AMACR deficiency; AMACR_HUMAN antibody CBAS4; Da1-8; EC 5.1.99.4; Macr1; Methylacyl CoA racemase alpha; RACE; RM; anti-AMACR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: AMACR/P504S monoclonal antibody detects endogenous levels of AMACR/P504S protein.
• Purity/Purification: ProG affinity purified.
• Form/Format: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 394

• Applicable Applications for anti-AMACR antibody: Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:2,000; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Recombinant full length protein of human AMACR.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of AMACR/P504S on Siha cell and human liver tissue lysates using anti-AMACR/P504S antibody at 1/1,000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.)

Related Product Information for anti-AMACR antibody

AMACR, is an enzyme that responsible for converting (2R)-methylacyl-CoA esters to their (2S)-methylacyl-CoA epimers and known substrates, including coenzyme A esters of pristanic acid (mostly derived from phytanic acid, a 3-methyl branched-chain fatty acid that is abundant in the diet) and bile acids derived from cholesterol. Both decreased and increased levels of the enzyme in humans are linked with diseases. Reduction of the protein level or activity results in the accumulation of (2R)-methyl fatty acids such as bile acids which causes neurological symptoms. Increased levels of AMACR protein concentration and activity are associated with prostate cancer, and the enzyme is used widely as a biomarker (known in cancer literature as P504S) in biopsy tissues. Increased levels of AMACR are also associated with some breast, colon, and other cancers, but it is unclear exactly what the role of AMACR is in these cancers. This AMACR antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalinfixed, paraffin-embedded tissue sections using IHC test methods.

NCBI and Uniprot Product Information

• NCBI GI #: 266456254
• NCBI GeneID: 23600
• NCBI Accession #: NP_001161067.1
• NCBI GenBank Nucleotide #: NP_001161067.1
• UniProt Accession #: Q9UHK6
• Molecular Weight: 42kDa
• NCBI Official Full Name: alpha-methylacyl-CoA racemase isoform 3
• NCBI Official Synonym Full Names: alpha-methylacyl-CoA racemase
• NCBI Official Symbol: AMACR
• NCBI Official Synonym Symbols: RM; RACE; CBAS4; P504S; AMACRD
• NCBI Protein Information: alpha-methylacyl-CoA racemase
• UniProt Protein Name: Alpha-methylacyl-CoA racemase
• Protein Family: Alpha-methylacyl-CoA racemase
• UniProt Gene Name: AMACR
• UniProt Entry Name: AMACR_HUMAN

NCBI Description

This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]