Rabbit anti-Human, Mouse AMACR Polyclonal Antibody | anti-AMACR antibody

AMACR Polyclonal Antibody

Cat. Num. AAA3012218

• Gene Names: AMACR; RM; RACE; CBAS4; P504S; AMACRD
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Protein affinity purified.
• Synonyms: AMACR; Polyclonal Antibody; AMACR Polyclonal Antibody; 2 arylpropionyl CoA epimerase; 2 methylacyl CoA racemase; 2-methylacyl-CoA racemase; Alpha methylacyl CoA racemase; Alpha methylacyl Coenzyme A racemase; Alpha methylacyl-CoA racemase deficiency; included; Alpha-methylacyl-CoA racemase; Amacr; AMACR deficiency; CBAS4; Da1-8; EC 5.1.99.4; Macr1; Methylacyl CoA racemase alpha; RACE; RM; anti-AMACR antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: AMACR polyclonal antibody detects endogenous levels of AMACR protein.
• Purity/Purification: Protein affinity purified.
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 394

• Applicable Applications for anti-AMACR antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500; ICC: 1:50-1:100; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: Recombinant protein.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of AMACR on different lysates using anti-AMACR antibody at 1/1,000 dilution. Positive control: Lane1: Mouse liver tissue Lane2: SiHaLane3: SW480Lane4: Human kidney tissue)

Immunocytochemistry (ICC)

(ICC staining AMACR in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-AMACR antibody

P504S, also known as AMACR (alpha-methylacyl-CoA racemase), 2-methylacyl-CoA racemase or RACE, is an enzyme belonging to the caiB/baiF CoA-transferase family. Localizing to the peroxisome and mitochondrion, P504S plays a role in the metabolism of branched-chain fatty acids and bile acid intermediates. More specifically, P504S catalyzes the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers which can then be degraded by peroxisomal beta-oxidation. Mutations in the gene encoding P504S can lead to AMACR deficiency, a disease characterized by increased concentrations of pristanic acid that is associated with adult onset sensory motor neuropathy, and/or CBAS4 (congenital bile acid synthesis defect type 4), a disorder characterized by intrahepatic cholestasis, absence of cholic acid from bile, neonatal jaundice and bile duct deficiency. In addition, P504S is overexpressed in prostate cancer and is believed to be functionally important for prostate cancer cell growth.

NCBI and Uniprot Product Information

• NCBI GI #: 266456254
• NCBI GeneID: 23600
• NCBI Accession #: NP_001161067.1
• NCBI GenBank Nucleotide #: NP_001161067.1
• UniProt Accession #: Q9UHK6; O09174
• Molecular Weight: 42kDa
• NCBI Official Full Name: alpha-methylacyl-CoA racemase isoform 3
• NCBI Official Synonym Full Names: alpha-methylacyl-CoA racemase
• NCBI Official Symbol: AMACR
• NCBI Official Synonym Symbols: RM; RACE; CBAS4; P504S; AMACRD
• NCBI Protein Information: alpha-methylacyl-CoA racemase
• UniProt Protein Name: Alpha-methylacyl-CoA racemase
• Protein Family: Alpha-methylacyl-CoA racemase
• UniProt Gene Name: AMACR
• UniProt Entry Name: AMACR_HUMAN

NCBI Description

This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]