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Immunohistochemistry (IHC) (Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-9) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)

Rabbit anti-Mouse, Rat IL-9 Polyclonal Antibody | anti-IL-9 antibody

IL-9 polyclonal antibody

Gene Names
Il9; P40; Il-9
Reactivity
Mouse, Rat
Applications
Immunohistochemistry
Purity
affinity purified by Protein A
Synonyms
IL-9; Polyclonal Antibody; IL-9 polyclonal antibody; Interleukin-9; IL9; Homolog of mouse T cell and mast cell growth factor 40; HP40; IL 9; Mast cell growth factor; MCGF; Megakaryoblast growth factor; p40 cytokine; p40 T cell and mast cell growth factor; p40 T-cell and mast cell growth factor; T cell growth factor 3; T cell growth factor p40; T-cell growth factor p40; Interleukin 9; TCGF 3; anti-IL-9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Specificity
IL-9 polyclonal Antibody detects endogenous levels of IL-9 protein.
Purity/Purification
affinity purified by Protein A
Form/Format
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-IL-9 antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:100-500
Immunogen
KLH conjugated synthetic peptide derived from mouse IL-9:21-120/144
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-9) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)

Immunohistochemistry (IHC) (Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-9) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)

Testing Data

(Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min; Incubation: Anti-IL-9 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C.)

Testing Data (Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min; Incubation: Anti-IL-9 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C.)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (Mouse mammary tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-9) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)

Immunohistochemistry (IHC) (Paraformaldehyde-fixed, paraffin embedded (Mouse mammary tumor); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-9) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.)
Related Product Information for anti-IL-9 antibody
The protein encoded by this gene is a cytokine that actsas a regulator of a variety of hematopoietic cells. This cytokinestimulates cell proliferation and prevents apoptosis. It functionsthrough the interleukin 9 receptor (IL9R), which activatesdifferent signal transducer and activator (STAT) proteins and thusconnects this cytokine to various biological processes. The geneencoding this cytokine has been identified as a candidate gene forasthma. Genetic studies on a mouse model of asthma demonstratedthat this cytokine is a determining factor in the pathogenesis ofbronchial hyperresponsiveness. [provided by RefSeq, Jul 2008].

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,075 Da
NCBI Official Full Name
interleukin-9
NCBI Official Synonym Full Names
interleukin 9
NCBI Official Symbol
Il9
NCBI Official Synonym Symbols
P40; Il-9
NCBI Protein Information
interleukin-9; T-cell growth factor P40; cytokine P40
UniProt Protein Name
Interleukin-9
UniProt Gene Name
Il9
UniProt Synonym Gene Names
IL-9
UniProt Entry Name
IL9_MOUSE

Uniprot Description

IL9: Supports IL-2 independent and IL-4 independent growth of helper T-cells. Belongs to the IL-7/IL-9 family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interleukin-9 receptor binding; cytokine activity

Biological Process: immune response; positive regulation of cell growth; positive regulation of interleukin-5 biosynthetic process

Research Articles on IL-9

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Product Notes

The IL-9 il9 (Catalog #AAA3013883) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL-9 polyclonal antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IL-9 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:100-500. Researchers should empirically determine the suitability of the IL-9 il9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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