Rabbit anti-Human KLF13 Polyclonal Antibody | anti-KLF13 antibody

Anti-KLF13 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100822

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: KLF13; Polyclonal Antibody; Anti-KLF13 Antibody; Rabbit Polyclonal; KLF13 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-BTEB3 Antibody; Anti-FKLF2 Antibody; Anti-NSLP1 Antibody; Anti-RFLAT-1 Antibody; Anti-RFLAT1 Antibody; anti-KLF13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human KLF13
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-KLF13 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 1-2uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human KLF13
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human KLF13, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(KLF13 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate1 uL anti-KLF13 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-KLF13 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 31 kDaObserved band size: 35 kDa)

Western Blot (WB)

(Anti-KLF13 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:31 kDaObserved band size:35 kDa)

Related Product Information for anti-KLF13 antibody

Kruppel-like factor 13 (KLF13), a member of the KLF family, is involved in the development of immunological diseases and tumor progression. KLF13 functions as a tumor suppressor protein in PCa, and the pharmacological activation of KLF13 might represent a potential approach for the treatment of prostate cancer. KLF13 is critical for the activation of the HPV productive life cycle and is likely involved in the initiation and progression of cervical cancer.

NCBI and Uniprot Product Information

• UniProt Accession #: Q9Y2Y9
• UniProt Protein Name: Krueppel-like factor 13
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF13
• UniProt Synonym Gene Names: BTEB3; NSLP1
• UniProt Entry Name: KLF13_HUMAN

Uniprot Description

KLF13: Represses transcription by binding to the BTE site, a GC-rich DNA element, in competition with the activator SP1. It also represses transcription by interacting with the corepressor Sin3A and HDAC1. Activates RANTES expression in T-cells. Belongs to the Sp1 C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 15q12

Cellular Component: nucleus

Molecular Function: protein binding; DNA binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; negative regulation of cell proliferation; negative regulation of erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter

Disease: Chromosome 15q13.3 Deletion Syndrome