Rabbit ASPSCR1 Polyclonal Antibody | anti-ASPSCR1 antibody

ASPSCR1 Polyclonal Antibody

Cat. Num. AAA3012131

• Gene Names: ASPSCR1; TUG; ASPL; ASPS; RCC17; UBXD9; UBXN9; ASPCR1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ASPSCR1; Polyclonal Antibody; ASPSCR1 Polyclonal Antibody; Tether containing UBX domain for GLUT4; Alveolar soft part sarcoma chromosomal region candidate gene 1 protein; Alveolar soft part sarcoma locus; Renal papillary cell carcinoma protein 17; UBX domain-containing protein 9; ASPL; RCC17; TUG; UBXD9; UBXN9; anti-ASPSCR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ASPSCR1 polyclonal antibody detects endogenous levels of ASPSCR1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 647

• Applicable Applications for anti-ASPSCR1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200; IP: 1:50-1:100
• Immunogen: Recombinant full length Human ASPSCR1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using ASPSCR1 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using ASPSCR1 antibody.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug ASPSCR1 antibody.)

Related Product Information for anti-ASPSCR1 antibody

The protein encoded by this gene contains a UBX domain and interacts with glucose transporter type 4 (GLUT4). This protein is a tether, which sequesters the GLUT4 in intracellular vesicles in muscle and fat cells in the absence of insulin, and redistributes the GLUT4 to the plasma membrane within minutes of insulin stimulation. Translocation t(X;17)(p11;q25) of this gene with transcription factor TFE3 gene results in a ASPSCR1-TFE3 fusion protein in alveolar soft part sarcoma and in renal cell carcinomas. Multiple alternatively spliced transcript variants have been found.

NCBI and Uniprot Product Information

• NCBI GI #: 353677961
• NCBI GeneID: 79058
• NCBI Accession #: NP_001238817.1
• NCBI GenBank Nucleotide #: NP_001238817.1
• UniProt Accession #: Q9BZE9
• Molecular Weight: ~ 80kDa
• NCBI Official Full Name: tether containing UBX domain for GLUT4 isoform 2
• NCBI Official Synonym Full Names: ASPSCR1 tether for SLC2A4, UBX domain containing
• NCBI Official Symbol: ASPSCR1
• NCBI Official Synonym Symbols: TUG; ASPL; ASPS; RCC17; UBXD9; UBXN9; ASPCR1
• NCBI Protein Information: tether containing UBX domain for GLUT4
• UniProt Protein Name: Tether containing UBX domain for GLUT4
• Protein Family: Tether containing UBX domain
• UniProt Gene Name: ASPSCR1
• UniProt Synonym Gene Names: ASPL; RCC17; TUG; UBXD9; UBXN9
• UniProt Entry Name: ASPC1_HUMAN

NCBI Description

The protein encoded by this gene contains a UBX domain and interacts with glucose transporter type 4 (GLUT4). This protein is a tether, which sequesters the GLUT4 in intracellular vesicles in muscle and fat cells in the absence of insulin, and redistributes the GLUT4 to the plasma membrane within minutes of insulin stimulation. Translocation t(X;17)(p11;q25) of this gene with transcription factor TFE3 gene results in a ASPSCR1-TFE3 fusion protein in alveolar soft part sarcoma and in renal cell carcinomas. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Oct 2011]

Uniprot Description

ASPSCR1: Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface. Interacts with GLUT4. Ubiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, peripheral; Vesicle; Oncoprotein

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; internal side of plasma membrane; ER-Golgi intermediate compartment membrane; extrinsic to membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; plasma membrane; endomembrane system; vesicle membrane; cytosol

Molecular Function: protein binding

Biological Process: intracellular protein transport; regulation of glucose import; glucose homeostasis

Disease: Alveolar Soft Part Sarcoma