Rabbit BIRC7 Polyclonal Antibody | anti-BIRC7 antibody

BIRC7 Polyclonal Antibody

Cat. Num. AAA3011773

• Gene Names: BIRC7; KIAP; LIVIN; MLIAP; RNF50; ML-IAP
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: BIRC7; Polyclonal Antibody; BIRC7 Polyclonal Antibody; Baculoviral IAP repeat-containing protein 7; Kidney inhibitor of apoptosis protein; KIAP; Livin; Melanoma inhibitor of apoptosis protein; ML-IAP; RING finger protein 50; UNQ5800/PRO19607/PRO21344; LIVIN; MLIAP; RNF50; anti-BIRC7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ML-IAP/BIRC7 polyclonal antibody detects endogenous levels of ML-IAP/BIRC7 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 280

• Applicable Applications for anti-BIRC7 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human ML-IAP/BIRC7.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using BIRC7 Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat liver using BIRC7 antibody.)

Related Product Information for anti-BIRC7 antibody

Inhibitor of apoptosis proteins (IAPs) contain conserved, unique aminoterminal baculovirus IAP repeats (BIRs) and usually a C-terminal RING finger domain. Immunoprecipitation and Western blot analysis indicate that ML-IAP, also known as melanoma inhibitor of apoptosis protein, kidney inhibitor of apoptosis protein (KIAP), livin or BIRC7, binds to caspase-3, -7, and -9, but only inhibits caspase-9. Additionally, ML-IAP physically interacts with SMAC through its BIR domain with a very high affinity and this interaction is very specific. The gene which encodes ML-IAP maps to human chromosome 20q13.3. There is controversy regarding the localization of this protein and its involvement in apoptosis, but it has been suggested that ML-IAP may play a complex role in the regulation of apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 11545910
• NCBI GeneID: 79444
• NCBI Accession #: NP_071444.1
• NCBI GenBank Nucleotide #: NP_071444.1
• UniProt Accession #: Q96CA5
• Molecular Weight: ~ 32kDa
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 7 isoform beta
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 7
• NCBI Official Symbol: BIRC7
• NCBI Official Synonym Symbols: KIAP; LIVIN; MLIAP; RNF50; ML-IAP
• NCBI Protein Information: baculoviral IAP repeat-containing protein 7
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 7
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: BIRC7
• UniProt Synonym Gene Names: KIAP; LIVIN; MLIAP; RNF50; KIAP; ML-IAP; Truncated livin; p30-Livin; tLivin
• UniProt Entry Name: BIRC7_HUMAN

NCBI Description

This gene encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jul 2013]