Rabbit DAP Polyclonal Antibody | anti-DAP antibody

DAP Polyclonal Antibody

Cat. Num. AAA3011389

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DAP; Polyclonal Antibody; DAP Polyclonal Antibody; DAP 1; Dap; DAP-1; DAP1_HUMAN; Death Associated Protein 1; Death associated protein; Death-associated protein 1; MGC99796; OTTHUMP00000161670; OTTHUMP00000221331; anti-DAP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DAP polyclonal antibody detects endogenous levels of DAP protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 204

• Applicable Applications for anti-DAP antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human DAP.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of DAP polyclonal antibody)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cells, using DAP polyclonal antibody)

Related Product Information for anti-DAP antibody

In contrast to growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis. Cellular response to FAS-L and TNF is mediated by structurally related receptors containing a conserved cytoplasmic region called the "death domain". DAP-1 (for death associated protein-1) interacts with the death domain of TNF-R1 and can trigger programmed cell death in a variety of cell lines, as well as suppress NFkappaB/Rel activity. DAP-3 is a positive mediator of programmed cell death; overexpressed intact full-length protein is required in order to induce apoptosis. During apotosis, the caspase-activated DAP-5 can mediate CAP-independent translation at least from its own internal ribosome entry site, thus resulting in a positive feedback loop responsible for the continuous translation of DAP-5. DAP-5 is also required for cellular differentiation, as it controls specific gene expression pathways.

NCBI and Uniprot Product Information

• NCBI GI #: 634743288
• NCBI Accession #: NP_001278892.1
• NCBI GenBank Nucleotide #: NP_001278892.1
• UniProt Accession #: P51397
• Molecular Weight: ~ 11kDa
• NCBI Official Full Name: death-associated protein 1 isoform 1
• UniProt Protein Name: Death-associated protein 1
• Protein Family: Dapper
• UniProt Gene Name: DAP
• UniProt Synonym Gene Names: DAP1; DAP-1
• UniProt Entry Name: DAP1_HUMAN

Uniprot Description

DAP: a 15 kDa, proline rich protein that possesses one SH3 binding motif. Negatively regulates autophagy and is involved in mediating interferon-gamma-induced cell death. Localizes in the cytoplasm, but the detailed mechanism of its proapoptotic function is unclear.

Protein type: Autophagy

Chromosomal Location of Human Ortholog: 5p15.2

Biological Process: apoptosis; caspase activation; cellular response to amino acid starvation; inhibition of NF-kappaB transcription factor; negative regulation of autophagy; negative regulation of transcription, DNA-dependent