Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human FSTL1 expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with FITC-conjugated anti-Human FSTL1. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Rabbit anti-Human FSTL1 Monoclonal Antibody | anti-FSTL1 antibody
Recombinant Anti-FSTL1 Antibody (FITC), Rabbit Monoclonal
Gene Names
FSTL1; FRP; FSL1; MIR198
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
FSTL1; Monoclonal Antibody; Recombinant Anti-FSTL1 Antibody (FITC); Rabbit Monoclonal; FSTL1 Antibody (FITC); Rabbit MAb; follistatin-like 1; Anti-FRP Antibody; Anti-FSL1 Antibody; Anti-MIR198 Antibody; anti-FSTL1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
009
Specificity
Human FSTL1
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
10ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-FSTL1 antibody
Flow Cytometry (FC/FACS/FCM)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human FSTL1 Protein
Conjugation
FITC
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human FSTL1 (rh FSTL1) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human FSTL1 expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with FITC-conjugated anti-Human FSTL1. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Human FSTL1 expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with FITC-conjugated anti-Human FSTL1. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-FSTL1 antibody
Follistatin-related protein 1 (FSTL1) is an extracellular glycoprotein whose functional significance in physiological and pathological processes is incompletely understood. Recently, we have shown that FSTL1 acts as a muscle-derived secreted factor that is up-regulated by Akt activation and ischemic stress and that FSTL1 exerts favorable actions on the heart and vasculature. Here, we sought to identify the receptor that mediates the cellular actions of FSTL1. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. FSTL1 is thought to be an autoantigen associated with rheumatoid arthritis. DIP2A functions as a novel receptor that mediates the cardiovascular protective effects of FSTL1. Experiment results have provided in vivo and in vitro evidence to demonstrate that Fstl1 modulates lung development and alveolar maturation, in part, through BMP4 signaling.
References
Rosenberg MI, et al. (2006) MyoD inhibits Fstl1 and Utrn expression by inducing transcription of miR-206. J Cell Biol. 175(1): 77-85.Ouchi N, et al. (2010) DIP2A functions as a FSTL1 receptor. J Biol Chem. 285(10): 7127-34.Geng Y, et al. (2011) Follistatin-like 1 (Fstl1) is a bone morphogenetic protein (BMP) 4 signaling antagonist in controlling mouse lung development. Proc Natl Acad Sci U S A. 108(17): 7058-63.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,228 Da
NCBI Official Full Name
follistatin-related protein 1
NCBI Official Synonym Full Names
follistatin-like 1
NCBI Official Symbol
FSTL1
NCBI Official Synonym Symbols
FRP; FSL1; MIR198
NCBI Protein Information
follistatin-related protein 1; follistatin-like protein 1
UniProt Protein Name
Follistatin-related protein 1
Protein Family
UniProt Gene Name
FSTL1
UniProt Synonym Gene Names
FRP
UniProt Entry Name
FSTL1_HUMAN
NCBI Description
This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. [provided by RefSeq, Jul 2008]
Uniprot Description
FSTL1: May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3q13.33
Cellular Component: extracellular space; extracellular region
Molecular Function: heparin binding; protein binding; calcium ion binding
Biological Process: BMP signaling pathway; response to starvation
Research Articles on FSTL1
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Product Notes
The FSTL1 fstl1 (Catalog #AAA8104337) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-FSTL1 Antibody (FITC), Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FSTL1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the FSTL1 fstl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FSTL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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