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Western Blot (WB) (Western Blot analysis of extracts of various cells, using CNOT8 antibody.)

Rabbit CNOT8 Polyclonal Antibody | anti-CNOT8 antibody

CNOT8 Polyclonal Antibody

Gene Names
CNOT8; CAF1; POP2; CALIF; Caf1b; hCAF1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
CNOT8; Polyclonal Antibody; CNOT8 Polyclonal Antibody; CAF1-like protein; CALIFp; CAF2; CCR4-associated factor 8; Caf1b; CALIF; POP2; anti-CNOT8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CNOT8 polyclonal antibody detects endogenous levels of CNOT8 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
292
Applicable Applications for anti-CNOT8 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
Recombinant protein of human CNOT8.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cells, using CNOT8 antibody.)

Western Blot (WB) (Western Blot analysis of extracts of various cells, using CNOT8 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophagus using CNOT8 antibody at dilution of 1:100 (x40 lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human esophagus using CNOT8 antibody at dilution of 1:100 (x40 lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse stomach using CNOT8 antibody at dilution of 1:100 (x40 lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse stomach using CNOT8 antibody at dilution of 1:100 (x40 lens).)
Related Product Information for anti-CNOT8 antibody
CNOT8 (CCR4-NOT transcription complex subunit 8), also known as CALIF or POP2, is a 292 amino acid protein that localizes to both the nucleus and the cytoplasm and functions as part of the CCR-NOT complex. Expressed ubiquitously, CNOT8 plays a role in transcriptional regulation for a diverse set of processes.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 34kDa
NCBI Official Full Name
CCR4-NOT transcription complex subunit 8 isoform 1
NCBI Official Synonym Full Names
CCR4-NOT transcription complex subunit 8
NCBI Official Symbol
CNOT8
NCBI Official Synonym Symbols
CAF1; POP2; CALIF; Caf1b; hCAF1
NCBI Protein Information
CCR4-NOT transcription complex subunit 8
UniProt Protein Name
CCR4-NOT transcription complex subunit 8
UniProt Gene Name
CNOT8
UniProt Synonym Gene Names
CALIF; POP2; CALIFp
UniProt Entry Name
CNOT8_HUMAN

Uniprot Description

CNOT8: Ubiquitous transcription factor required for a diverse set of processes. The CCR4-NOT complex functions as general transcription regulation complex. Belongs to the CAF1 family.

Protein type: EC 3.1.13.4

Chromosomal Location of Human Ortholog: 5q31-q33

Cellular Component: intracellular; cytosol; nucleus; CCR4-NOT complex

Molecular Function: protein binding; 3'-5'-exoribonuclease activity; poly(A)-specific ribonuclease activity; RNA binding; metal ion binding; transcription factor activity

Biological Process: regulation of translation; negative regulation of cell proliferation; poly(A) tail shortening; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of cell proliferation; gene expression; miRNA-mediated gene silencing; mRNA catabolic process, deadenylation-dependent decay

Research Articles on CNOT8

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Product Notes

The CNOT8 cnot8 (Catalog #AAA3009020) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CNOT8 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CNOT8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the CNOT8 cnot8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CNOT8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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