Rabbit STXBP1 Polyclonal Antibody | anti-STXBP1 antibody

STXBP1 polyclonal antibody

Cat. Num. AAA3007750

• Gene Names: STXBP1; P67; NSEC1; UNC18; N-Sec1; RBSEC1; unc-18A; unc18-1; MUNC18-1
• Reactivity: Human, Mouse, Rabbit
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: STXBP1; Polyclonal Antibody; STXBP1 polyclonal antibody; Syntaxin-binding protein 1; MUNC18-1; N-Sec1; Protein unc-18 homolog 1; Unc18-1; Protein unc-18 homolog A; Unc-18A; p67; UNC18A; anti-STXBP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: STXBP1 polyclonal antibody detects endogenous levels of STXBP1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
• Sequence Length: 594

• Applicable Applications for anti-STXBP1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to Human STXBP1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of STXBP1 polyclonal antibody at 1:500 dilutionLane1:U-87MG whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:SHSY5Y whole cell lysate (40ug) Lane4:K562 whole cell lysate (40ug) Lane5:RAW264. 7 whole cell lysate (40ug) Lane6:The Brain tissue lysate of Mouse (40ug) Lane7:The Brain tissue lysate of Rat (40ug))

Related Product Information for anti-STXBP1 antibody

Munc18-1 belongs to the Munc18 protein family that also includes Munc18-2 and Munc18-3. These proteins are highly conserved at the amino acid sequence level and play a critical role in membrane fusion. Munc18 proteins control SNARE complex formation and interact with syntaxin. In Munc18-1 knockout mice, neurotransmitter release is completely abolished. In addition, Munc18-1 acts as a molecular chaperone for syntaxin-1, allowing for formation of the SNARE complex at the plasma membrane. Munc18-1 also acts as a regulator of vesicle docking during exocytosis.

NCBI and Uniprot Product Information

• NCBI GI #: 73760415
• NCBI GeneID: 6812
• NCBI Accession #: NP_001027392.1
• NCBI GenBank Nucleotide #: NP_001027392.1
• UniProt Accession #: P61764
• Molecular Weight: ~ 70kDa
• NCBI Official Full Name: syntaxin-binding protein 1 isoform b
• NCBI Official Synonym Full Names: syntaxin binding protein 1
• NCBI Official Symbol: STXBP1
• NCBI Official Synonym Symbols: P67; NSEC1; UNC18; N-Sec1; RBSEC1; unc-18A; unc18-1; MUNC18-1
• NCBI Protein Information: syntaxin-binding protein 1
• UniProt Protein Name: Syntaxin-binding protein 1
• Protein Family: Syntaxin-binding protein
• UniProt Gene Name: STXBP1
• UniProt Synonym Gene Names: UNC18A; Unc18-1; Unc-18A
• UniProt Entry Name: STXB1_HUMAN

NCBI Description

This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010]

Uniprot Description

STXBP1: may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. Two splice-variant isoforms have been described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleoplasm; protein complex; mitochondrion; cytoplasm; plasma membrane; cytosol

Molecular Function: identical protein binding; protein domain specific binding; SNARE binding; syntaxin binding; syntaxin-1 binding; protein N-terminus binding; protein kinase binding

Biological Process: protein stabilization; synaptic vesicle maturation; neurotransmitter secretion; regulation of synaptic vesicle fusion to presynaptic membrane; positive regulation of calcium ion-dependent exocytosis; synaptic transmission; protein transport; platelet degranulation; axon target recognition; glutamate secretion; energy reserve metabolic process; neuromuscular synaptic transmission; negative regulation of neuron apoptosis; negative regulation of synaptic transmission, GABAergic; regulation of insulin secretion; vesicle docking during exocytosis

Disease: Epileptic Encephalopathy, Early Infantile, 4