Human STXBP1 (Syntaxin-Binding Protein 1) ELISA Kit | STXBP1 elisa kit

Human STXBP1 (Syntaxin-Binding Protein 1) ELISA Kit

Cat. Num. AAA7607917

• Gene Names: STXBP1; P67; NSEC1; UNC18; N-Sec1; RBSEC1; unc-18A; unc18-1; MUNC18-1
• Reactivity: Human
• Synonyms: STXBP1 (Syntaxin-Binding Protein 1); Human STXBP1 (Syntaxin-Binding Protein 1) ELISA Kit; EIEE4; MUNC18 1; N Sec1; p67; Protein unc 18 homolog 1; Protein unc 18 homolog A; RBSEC1; STXBP1; syntaxin binding protein 1; Unc 18A; UNC18; Unc18 1; UNC18A; STXBP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of STXBP1. No significant cross-reactivity or interference between STXBP1 and analogues was observed.
• Sequence Length: 594

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for STXBP1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 73760415
• NCBI GeneID: 6812
• NCBI Accession #: NP_001027392.1
• NCBI GenBank Nucleotide #: NP_001027392.1
• UniProt Accession #: P61764
• NCBI Official Full Name: syntaxin-binding protein 1 isoform b
• NCBI Official Synonym Full Names: syntaxin binding protein 1
• NCBI Official Symbol: STXBP1
• NCBI Official Synonym Symbols: P67; NSEC1; UNC18; N-Sec1; RBSEC1; unc-18A; unc18-1; MUNC18-1
• NCBI Protein Information: syntaxin-binding protein 1
• UniProt Protein Name: Syntaxin-binding protein 1
• Protein Family: Syntaxin-binding protein
• UniProt Gene Name: STXBP1
• UniProt Synonym Gene Names: UNC18A; Unc18-1; Unc-18A
• UniProt Entry Name: STXB1_HUMAN

NCBI Description

This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010]

Uniprot Description

STXBP1: may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. Two splice-variant isoforms have been described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: nucleoplasm; protein complex; mitochondrion; cytoplasm; plasma membrane; cytosol

Molecular Function: identical protein binding; protein domain specific binding; SNARE binding; syntaxin binding; syntaxin-1 binding; protein N-terminus binding; protein kinase binding

Biological Process: protein stabilization; synaptic vesicle maturation; neurotransmitter secretion; regulation of synaptic vesicle fusion to presynaptic membrane; positive regulation of calcium ion-dependent exocytosis; synaptic transmission; protein transport; platelet degranulation; axon target recognition; glutamate secretion; energy reserve metabolic process; neuromuscular synaptic transmission; negative regulation of neuron apoptosis; negative regulation of synaptic transmission, GABAergic; regulation of insulin secretion; vesicle docking during exocytosis

Disease: Epileptic Encephalopathy, Early Infantile, 4