Rabbit SLC28A1 Polyclonal Antibody | anti-SLC28A1 antibody

SLC28A1 Polyclonal Antibody

Cat. Num. AAA3006491

• Gene Names: SLC28A1; CNT1; HCNT1; URCTU
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SLC28A1; Polyclonal Antibody; SLC28A1 Polyclonal Antibody; Sodium/nucleoside cotransporter 1; Concentrative nucleoside transporter 1; CNT 1; hCNT1; Na(+)/nucleoside cotransporter 1; Sodium-coupled nucleoside transporter 1; Solute carrier family 28 member 1; CNT1; anti-SLC28A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SLC28A1 polyclonal antibody detects endogenous levels of SLC28A1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 649

• Applicable Applications for anti-SLC28A1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human SLC28A1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SLC28A1 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Mouse brain tissue lysateLane3:H9C2 whole cell lysate)

Related Product Information for anti-SLC28A1 antibody

Nucleosides play a role in signaling in several physiologic systems, and synthetic analogs of natural nucleosides are often used to treat neoplastic and viral diseases. Plasma membrane transport of nucleosides is mediated by equilibrative and concentrative nucleoside transporters, which may have specificity for purines or pyrimidines. The deduced human 650 amino acid concentrative nucleoside transporter 1 (CNT1) protein is 83% identical to the rat protein and is expressed in the intestine, kidney and liver. CNT1, also designated solute carrier family 28 (sodium-coupled nucleoside transporter), member 1 (SLC28A1), expedites sodium-dependent fluxes of uridine, azidodeoxythymidine (AZT) and adenosine, but not of guanosine or deoxyadenosine, which undergo net renal secretion. CNT1 activity may serve as a putative mechanism for renal reabsorption of physiologic nucleosides and synthetic nucleoside drugs.

NCBI and Uniprot Product Information

• NCBI GI #: 567757527
• NCBI GeneID: 9154
• NCBI Accession #: NP_001274691.1
• NCBI GenBank Nucleotide #: NP_001274691.1
• UniProt Accession #: O00337
• Molecular Weight: ~ 71kDa
• NCBI Official Full Name: sodium/nucleoside cotransporter 1 isoform 1
• NCBI Official Synonym Full Names: solute carrier family 28 member 1
• NCBI Official Symbol: SLC28A1
• NCBI Official Synonym Symbols: CNT1; HCNT1; URCTU
• NCBI Protein Information: sodium/nucleoside cotransporter 1
• UniProt Protein Name: Sodium/nucleoside cotransporter 1
• Protein Family: Sodium/nucleoside cotransporter
• UniProt Gene Name: SLC28A1
• UniProt Synonym Gene Names: CNT1; CNT 1; hCNT1
• UniProt Entry Name: S28A1_HUMAN

Uniprot Description

SLC28A1: Sodium-dependent and pyrimidine-selective. Exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit) (selective for pyrimidine nucleosides and adenosine). It also transports the antiviral pyrimidine nucleoside analogs 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxycytidine (ddC). It may be involved in the intestinal absorption and renal handling of pyrimidine nucleoside analogs used to treat acquired immunodeficiency syndrome (AIDS). It has the following selective inhibition: adenosine, thymidine, cytidine, uridine >> guanosine, inosine. Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter, SLC family; Transporter

Chromosomal Location of Human Ortholog: 15q25.3

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: nucleoside transmembrane transporter activity; nucleoside:sodium symporter activity; nucleoside binding

Biological Process: nucleoside transport; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; transmembrane transport