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Western Blot (WB) (Western blot analysis SLC28A1 using Jurkat whole cell lysates)

Rabbit anti-Human, Mouse SLC28A1 Polyclonal Antibody | anti-SLC28A1 antibody

SLC28A1 Antibody

Gene Names
SLC28A1; CNT1; HCNT1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SLC28A1; Polyclonal Antibody; SLC28A1 Antibody; CNT 1; CNT1; Concentrative nucleoside transporter 1; hCNT1; Na(+)/nucleoside cotransporter 1; S28A1_HUMAN; Sodium-coupled nucleoside transporter 1; Sodium/nucleoside cotransporter 1; solute carrier family 28 concentrative nucleoside transporter member 1; Solute carrier family 28 member 1; anti-SLC28A1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
SLC28A1 antibody detects endogenous levels of total SLC28A1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
649
Applicable Applications for anti-SLC28A1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
Immunogen
A synthesized peptide derived from human SLC28A1
Subcellular Location
Cell Membrane
Tissue Specificity
Expressed in kidney.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis SLC28A1 using Jurkat whole cell lysates)

Western Blot (WB) (Western blot analysis SLC28A1 using Jurkat whole cell lysates)

Immunohistochemistry (IHC)

(MBS9610255 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9610255 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-SLC28A1 antibody
Function: Sodium-dependent and pyrimidine-selective. Exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit) (selective for pyrimidine nucleosides and adenosine). It also transports the antiviral pyrimidine nucleoside analogs 3'-azido-3'-deoxythymidine (AZT) and 2', 3'-dideoxycytidine (ddC). It may be involved in the intestinal absorption and renal handling of pyrimidine nucleoside analogs used to treat acquired immunodeficiency syndrome (AIDS). It has the following selective inhibition: adenosine, thymidine, cytidine, uridine > > guanosine, inosine.
Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 72 kDa
Predicted: 72 kDa
NCBI Official Full Name
sodium/nucleoside cotransporter 1 isoform 1
NCBI Official Synonym Full Names
solute carrier family 28 member 1
NCBI Official Symbol
SLC28A1
NCBI Official Synonym Symbols
CNT1; HCNT1
NCBI Protein Information
sodium/nucleoside cotransporter 1
UniProt Protein Name
Sodium/nucleoside cotransporter 1
UniProt Gene Name
SLC28A1
UniProt Synonym Gene Names
CNT1; CNT 1; hCNT1

Uniprot Description

Sodium-dependent and pyrimidine-selective. Exhibits the transport characteristics of the nucleoside transport system cit or N2 subtype (N2/cit) (selective for pyrimidine nucleosides and adenosine). It also transports the antiviral pyrimidine nucleoside analogs 3'-azido-3'-deoxythymidine (AZT) and 2',3'-dideoxycytidine (ddC). It may be involved in the intestinal absorption and renal handling of pyrimidine nucleoside analogs used to treat acquired immunodeficiency syndrome (AIDS). It has the following selective inhibition: adenosine, thymidine, cytidine, uridine >> guanosine, inosine.

Research Articles on SLC28A1

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Product Notes

The SLC28A1 slc28a1 (Catalog #AAA9610255) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SLC28A1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SLC28A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the SLC28A1 slc28a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SLC28A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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