Human Transcriptional Intermediary Factor 1 Gamma (TIF1g) ELISA Kit | TIF1g elisa kit

Human Transcriptional Intermediary Factor 1 Gamma (TIF1g) ELISA Kit

Cat. Num. AAA2122008

• Gene Names: TRIM33; ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA
• Reactivity: Human
• Synonyms: Transcriptional Intermediary Factor 1 Gamma (TIF1g); Human Transcriptional Intermediary Factor 1 Gamma (TIF1g) ELISA Kit; TRIM33; PTC7; RFG7; TF1G; TIF1GAMMA; TIFGAMMA; Tripartite Motif-Containing 33; Ret-Fused Gene 7; RET-fused gene 7 protein; Ectodermin homolog; TIF1g elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transcriptional Intermediary Factor 1 Gamma (TIF1g).; No significant cross-reactivity or interference between Transcriptional Intermediary Factor 1 Gamma (TIF1g) and analogues was observed.
• Sequence Length: 1127

• Assay Type: Double-antibody Sandwich
• Sample: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Dectection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.056ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transcriptional Intermediary Factor 1 Gamma (TIF1g) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transcriptional Intermediary Factor 1 Gamma (TIF1g) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TIF1g elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcriptional Intermediary Factor 1 Gamma (TIF1g). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcriptional Intermediary Factor 1 Gamma (TIF1g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcriptional Intermediary Factor 1 Gamma (TIF1g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transcriptional Intermediary Factor 1 Gamma (TIF1g) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TIF1g elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 74027249
• NCBI GeneID: 51592
• NCBI Accession #: NP_056990.3
• NCBI GenBank Nucleotide #: NP_056990.3
• UniProt Accession #: Q9UPN9
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM33 isoform alpha
• NCBI Official Synonym Full Names: tripartite motif containing 33
• NCBI Official Symbol: TRIM33
• NCBI Official Synonym Symbols: ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM33
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM33
• UniProt Gene Name: TRIM33
• UniProt Synonym Gene Names: KIAA1113; RFG7; TIF1G; Protein Rfg7; TIF1-gamma
• UniProt Entry Name: TRI33_HUMAN

NCBI Description

The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TIF1G: an atypical protein kinase that functions as a transcriptional repressor. Translocation with RET generates the TRIM33/RET (PTC7) oncogene. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Two splice variant isoforms have been described.

Protein type: Ubiquitin ligase; Transcription, coactivator/corepressor; EC 6.3.2.19; Protein kinase, atypical; Ubiquitin conjugating system; EC 6.3.2.-; Ligase; Kinase, protein; ATYPICAL group; TIF1 family

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination; gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of BMP signaling pathway

Disease: Thyroid Carcinoma, Papillary