Testing Data
(F4/80 expression on macrophages in mouse liver. Staining of frozen tissue section with MBS645883.)
Rat anti-Human, Mouse F4/80, Pan Macrophages Monoclonal Antibody
F4/80, Pan Macrophages (Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-like Module-containing Mucin-like Hormone Receptor-like 1 Precursor, EGF-TM7, EMR1 Hormone Receptor, F4/80, Gpf480, Ly71, TM7LN3) (PE)
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
F4/80; Pan Macrophages; Monoclonal Antibody; Pan Macrophages (Cell Surface Glycoprotein EMR1; Cell Surface Glycoprotein F4/80; DD7A5-7; EGF-like Module-containing Mucin-like Hormone Receptor-like 1 Precursor; EGF-TM7; EMR1 Hormone Receptor; Gpf480; Ly71; TM7LN3) (PE); Anti -F4/80; anti-F4/80; Pan Macrophages antibody
Host
Rat
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
13A47
Specificity
Recognizes mouse F4/80, a 125kD extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. Does not react with mouse granulocytes, mast cells, platelets, lymphocytes fibroblasts and endothelial cells. Species Crossreactivity: human (heart macrophages).
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-F4/80, Pan Macrophages antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot.
Dilution: Flow Cytometry: 10ul/250,000 cells in a 100ul total staining volume
Immunohistochemistry (Frozen): tissue embedded in OCT Tissue Tec; fixed with acetone for 10min at RT; incubation with 0.02M sodium azide in PBS containing 0.1% H2O2 for 10 min at RT to destroy endogenous peroxidase; spleen as positive control
Immunohistochemistry (Paraffin): fixation in 10% neutral buffered formalin for 24h; blocking with non-immunized goat serum; microwaved for 6min in citrate buffer; splenic macrophages as positive control
Western Blot: Mouse bone-marrow derived macrophages; non-reduced; ~125kD; reduction with 2-mercaptoethanol destroys BM8 antigen
Dilution: Flow Cytometry: 10ul/250,000 cells in a 100ul total staining volume
Immunohistochemistry (Frozen): tissue embedded in OCT Tissue Tec; fixed with acetone for 10min at RT; incubation with 0.02M sodium azide in PBS containing 0.1% H2O2 for 10 min at RT to destroy endogenous peroxidase; spleen as positive control
Immunohistochemistry (Paraffin): fixation in 10% neutral buffered formalin for 24h; blocking with non-immunized goat serum; microwaved for 6min in citrate buffer; splenic macrophages as positive control
Western Blot: Mouse bone-marrow derived macrophages; non-reduced; ~125kD; reduction with 2-mercaptoethanol destroys BM8 antigen
Immunogen
BALB/c macrophages obtained from 14-day-old bone marrow cell cultures
Positive Control
Mouse macrophages
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4 degree C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Testing Data
(F4/80 expression on macrophages in mouse liver. Staining of frozen tissue section with MBS645883.)
Testing Data
(F4/80 expression on macrophages in mouse liver. Staining of frozen tissue section with MBS645883.)
Related Product Information for anti-F4/80, Pan Macrophages antibody
Murine F4/80 glycoprotein contains seven-transmembrane (TM7) regions, which anchor the protein in the cell membrane, and thereby shows similarity in this region to G-protein-coupled receptors. The F4/80 molecule shares overall structural homology to other members of the epidermal growth factor (EGF)-TM7 family. The antigen is detected on tissue fixed macrophages in all organs tested so far (spleen, lymph nodes, thymus, liver, skin). It is also present on Langerhans cells in the skin and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes and thrombocytes. The expression of F4/80 increases upon maturation of macrophage precursors in bone marrow and blood as well as in ontogeny. This monoclonal antibody is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. Furthermore it is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.
Product Categories/Family for anti-F4/80, Pan Macrophages antibody
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Product Notes
The F4/80, Pan Macrophages (Catalog #AAA645883) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The F4/80, Pan Macrophages (Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-like Module-containing Mucin-like Hormone Receptor-like 1 Precursor, EGF-TM7, EMR1 Hormone Receptor, F4/80, Gpf480, Ly71, TM7LN3) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's F4/80, Pan Macrophages can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Dilution: Flow Cytometry: 10ul/250,000 cells in a 100ul total staining volume Immunohistochemistry (Frozen): tissue embedded in OCT Tissue Tec; fixed with acetone for 10min at RT; incubation with 0.02M sodium azide in PBS containing 0.1% H2O2 for 10 min at RT to destroy endogenous peroxidase; spleen as positive control Immunohistochemistry (Paraffin): fixation in 10% neutral buffered formalin for 24h; blocking with non-immunized goat serum; microwaved for 6min in citrate buffer; splenic macrophages as positive control Western Blot: Mouse bone-marrow derived macrophages; non-reduced; ~125kD; reduction with 2-mercaptoethanol destroys BM8 antigen. Researchers should empirically determine the suitability of the F4/80, Pan Macrophages for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "F4/80, Pan Macrophages, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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