Rabbit Eg5  Polyclonal Antibody | anti-Eg5  antibody

Eg5 (Phospho-T926) Polyclonal Antibody

Cat. Num. AAA3005032

• Gene Names: KIF11; EG5; HKSP; KNSL1; MCLMR; TRIP5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Eg5 ; Polyclonal Antibody; Eg5 (Phospho-T926) Polyclonal Antibody; Kinesin-like protein KIF11; Kinesin-like protein 1; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5; KIF11; EG5; KNSL1; TRIP5; anti-Eg5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-Eg5 (T926) pAb detects endogenous levels of Eg5 protein only when phosphorylated at Thr926.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1056

• Applicable Applications for anti-Eg5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic phosphopeptide derived from human Eg5 around the phosphorylation site of Threonine 926
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-Eg5  (T926)  pAb at 1:500 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:CT-26 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:LOVO whole cell lysate (40ug) Lane6:MCF-7 whole cell lysate (40ug))

Testing Data

Related Product Information for anti-Eg5  antibody

Eg5 (also called kinesin-like protein 11 or Kif11) belongs to the kinesin-like family of motor proteins important in chromosome positioning, centrosome separation, and mitotic spindle formation. Phosphorylation of Eg5 by mitotic kinases regulates its activity by modulating its association with microtubules. Because anti-mitotic chemotherapeutic drugs, such as taxanes, target microtubules and have pleiotropic and sometimes toxic effects, drugs that target microtubule-associated proteins such as Eg5 are currently in development.

NCBI and Uniprot Product Information

• NCBI GI #: 13699824
• NCBI GeneID: 3832
• NCBI Accession #: NP_004514.2
• NCBI GenBank Nucleotide #: NP_004514.2
• UniProt Accession #: P52732
• Molecular Weight: ~ 120kDa
• NCBI Official Full Name: kinesin-like protein KIF11
• NCBI Official Synonym Full Names: kinesin family member 11
• NCBI Official Symbol: KIF11
• NCBI Official Synonym Symbols: EG5; HKSP; KNSL1; MCLMR; TRIP5
• NCBI Protein Information: kinesin-like protein KIF11
• UniProt Protein Name: Kinesin-like protein KIF11
• UniProt Gene Name: KIF11
• UniProt Synonym Gene Names: EG5; KNSL1; TRIP5; TR-interacting protein 5; TRIP-5
• UniProt Entry Name: KIF11_HUMAN

NCBI Description

This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq, Jul 2008]

Uniprot Description

KIF11: a cytoskeletal protein that belongs to the kinesin-like protein family. BimC subfamily. Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Plays a role in chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. Phosphorylation regulates its association with the spindle apparatus.

Protein type: Microtubule-binding; Motor

Chromosomal Location of Human Ortholog: 10q24.1

Cellular Component: spindle pole; kinesin complex; microtubule; membrane; spindle microtubule; cytoplasm; spindle; cytosol

Molecular Function: plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; protein complex binding; microtubule motor activity; protein kinase binding; ATP binding

Biological Process: mitosis; mitotic spindle organization and biogenesis; mitotic centrosome separation; cell division; metabolic process; regulation of mitotic centrosome separation; antigen processing and presentation of exogenous peptide antigen via MHC class II; blood coagulation; spindle organization and biogenesis; microtubule-based movement; chromosome segregation

Disease: Microcephaly With Or Without Chorioretinopathy, Lymphedema, Or Mental Retardation