Mouse KIF11/Eg5 Monoclonal Antibody | anti-KIF11/Eg5 antibody

KIF11/Eg5 (Central region) Antibody

Cat. Num. AAA474414

• Gene Names: KIF11; EG5; HKSP; KNSL1; MCLMR; TRIP5
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, ELISA
• Synonyms: KIF11/Eg5; Monoclonal Antibody; KIF11/Eg5 (Central region) Antibody; KIF11/Eg5 (Central region); anti-KIF11/Eg5 antibody

• Host: Mouse
• Reactivity: Human, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: M293
• Specificity: This antibody detects a 120kDa* protein corresponding to the molecular mass of KIF11 on SDS-PAGE immunoblots of human Jurkat cells.
• Form/Format: Mouse monoclonal, protein G purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-KIF11/Eg5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA: 1:2000; WB: 1:1000
• Immunogen: Clone (M293) was generated from a sequence corresponding to amino acids near the coiled-coil region of human KIF11. This sequence has high homology to rat and mouse KIF11.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

Western Blot (WB)

(Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-KIF11 (Central region) antibody at 1:250 (lane 1) and 1:1000 (lane 2).)

Related Product Information for anti-KIF11/Eg5 antibody

Microtubule-based kinesin superfamily motors move along microtubles during an array of cellular events, such as intracellular transport, mitosis, cytoskeletal rearrangement, and signal transduction. KIF11 (Eg5/Trip5/KNSL1/HKSP) is a Kinesin-5 family member with a conserved N-terminal catalytic motor domain followed by a neck linker leading to a coiled-coil stalk. This motor protein walks processively toward the plus-end of microtubules. KIF11 forms a homotetramer that includes a dimer in a parallel coiled-coil form complexed with a second dimer in an anti-parallel coiled-coil tetramer with four motor domains. This allows KIF11 to crosslink two adjacent microtubules such that each dimeric motor unit interacts with a single protofilament on each microtubule. KIF11 plays an essential role in the proper separation of spindle poles and the correct formation of bipolar mitotic spindle during mitosis. Inhibition of this protein results in cells apoptosis followed by mitotic arrest and the formation of characteristic monoaster spindles. Thus, KIF11 is an attractive anticancer therapeutic target.

References

Zhang Y. & Xu W. (2008). Anticancer Agents Med Chem. 8(6):698.
Valentine M.T. & Gilbert S.P. (2007) Curr Opin Cell Biol. 19(1):75.

NCBI and Uniprot Product Information

• NCBI GI #: 13699824
• NCBI GeneID: 3832
• NCBI Accession #: NP_004514.2
• NCBI GenBank Nucleotide #: NM_004523.3
• UniProt Accession #: P52732; Q15716; Q5VWX0; A0AV49; B2RMV3
• Molecular Weight: 119,159 Da
• NCBI Official Full Name: kinesin-like protein KIF11
• NCBI Official Synonym Full Names: kinesin family member 11
• NCBI Official Symbol: KIF11
• NCBI Official Synonym Symbols: EG5; HKSP; KNSL1; MCLMR; TRIP5
• NCBI Protein Information: kinesin-like protein KIF11; TRIP-5; kinesin-like protein 1; TR-interacting protein 5; kinesin-like spindle protein HKSP; kinesin-related motor protein Eg5; thyroid receptor-interacting protein 5
• UniProt Protein Name: Kinesin-like protein KIF11
• UniProt Gene Name: KIF11
• UniProt Synonym Gene Names: EG5; KNSL1; TRIP5; TR-interacting protein 5; TRIP-5
• UniProt Entry Name: KIF11_HUMAN

NCBI Description

This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. [provided by RefSeq, Jul 2008]

Uniprot Description

KIF11: a cytoskeletal protein that belongs to the kinesin-like protein family. BimC subfamily. Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Plays a role in chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. Phosphorylation regulates its association with the spindle apparatus.

Protein type: Microtubule-binding; Motor

Chromosomal Location of Human Ortholog: 10q24.1

Cellular Component: spindle pole; kinesin complex; microtubule; membrane; spindle microtubule; cytoplasm; spindle; cytosol

Molecular Function: plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; protein complex binding; microtubule motor activity; protein kinase binding; ATP binding

Biological Process: mitosis; mitotic spindle organization and biogenesis; mitotic centrosome separation; cell division; metabolic process; regulation of mitotic centrosome separation; antigen processing and presentation of exogenous peptide antigen via MHC class II; blood coagulation; spindle organization and biogenesis; microtubule-based movement; chromosome segregation

Disease: Microcephaly With Or Without Chorioretinopathy, Lymphedema, Or Mental Retardation