Rabbit ACAT1 Polyclonal Antibody | anti-ACAT1 antibody

ACAT1 (K266) Polyclonal Antibody

Cat. Num. AAA3004312

• Gene Names: ACAT1; T2; MAT; ACAT; THIL
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ACAT1; Polyclonal Antibody; ACAT1 (K266) Polyclonal Antibody; Acetyl-CoA acetyltransferase; mitochondrial; Acetoacetyl-CoA thiolase; ACAT; MAT; ACAT-1; anti-ACAT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ACAT1 (K266) polyclonal antibody detects endogenous levels of ACAT1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 427

• Applicable Applications for anti-ACAT1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 235-286 of Human ACAT1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ACAT-1 (K266) pAb at 1:1000 dilutionLane1:HEK293T whole cell lysate (20ug) Lane2:HepG2 whole cell lysate (20ug) Lane3:The Lung tissue lysate of Rat (20ug) Lane4:The Lung tissue lysate of Mouse (10ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ACAT1 (K266) pA bin paraffin-embedded human liver carcinoma tissue at 1:50,showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ACAT1 (K266) pA bin paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ACAT1 (K266) pA bin paraffin-embedded human rectum carcinoma tissue at 1:50,showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Western Blot (WB)

(Western Blot (WB) analysis of ACAT1 (K266) pAb at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:SP2/0 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug) Lane6:NIH-3T3 whole cell lysate (40ug))

Related Product Information for anti-ACAT1 antibody

ACAT-1 (acetyl-Coenzyme A acetyltransferase 1) is a mitochondrial enzyme involved in the formation and degradation of ketone bodies and is necessary for the proper metabolic processing of isoleucine. Rare defects in the gene encoding ACAT-1 lead to beta-ketothiolase deficiency, which is characterized by ketoacidotic attacks. ACAT-2 (acetyl-Coenzyme A acetyltransferase 2) is considered a cytosolic protein and is crucial for cholesterol synthesis. Specifically, both Acetoacetyl-CoA specific thiolases, ACAT-1 and ACAT-2 catalyze the formation of acetoacetyl-CoA from two acetyl-CoA molecules. These enzymes are also capable of the reverse reaction, the cleavage of acetoacetyl-CoA into two acetyl-CoA molecules.

NCBI and Uniprot Product Information

• NCBI GI #: 4557237
• NCBI GeneID: 38
• NCBI Accession #: NP_000010.1
• NCBI GenBank Nucleotide #: NP_000010.1
• UniProt Accession #: P24752
• Molecular Weight: ~ 45kDa
• NCBI Official Full Name: acetyl-CoA acetyltransferase, mitochondrial
• NCBI Official Synonym Full Names: acetyl-CoA acetyltransferase 1
• NCBI Official Symbol: ACAT1
• NCBI Official Synonym Symbols: T2; MAT; ACAT; THIL
• NCBI Protein Information: acetyl-CoA acetyltransferase, mitochondrial
• UniProt Protein Name: Acetyl-CoA acetyltransferase, mitochondrial
• UniProt Gene Name: ACAT1
• UniProt Synonym Gene Names: ACAT; MAT
• UniProt Entry Name: THIL_HUMAN

NCBI Description

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]

Uniprot Description

ACAT1: Plays a major role in ketone body metabolism. Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD); also known as alpha- methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3- hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. Belongs to the thiolase family.

Protein type: Mitochondrial; Lipid Metabolism - synthesis and degradation of ketone bodies; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - lysine degradation; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - butanoate; EC 2.3.1.9; Lipid Metabolism - fatty acid; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Acetyltransferase; Amino Acid Metabolism - tryptophan

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: protein homodimerization activity; enzyme binding; acetyl-CoA C-acetyltransferase activity; metal ion binding; coenzyme binding

Biological Process: response to starvation; ketone body catabolic process; response to hormone stimulus; ketone body biosynthetic process; branched chain family amino acid catabolic process; cellular lipid metabolic process; brain development; ketone body metabolic process; liver development; response to organic cyclic substance; protein homooligomerization

Disease: Alpha-methylacetoacetic Aciduria