Rabbit E2F4/E2F5 Polyclonal Antibody | anti-E2F4 antibody

E2F4/E2F5 (L50/83) Polyclonal Antibody

Cat. Num. AAA3000464

• Gene Names: E2F4; E2F-4
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: E2F4/E2F5; Polyclonal Antibody; E2F4/E2F5 (L50/83) Polyclonal Antibody; Transcription factor E2F4; E2F-4; E2F4; Transcription factor E2F5; E2F-5; E2F5; anti-E2F4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: E2F4/E2F5 (L50/83) polyclonal antibody detects endogenous levels of E2F4/E2F5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 413

• Applicable Applications for anti-E2F4 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 18-72 of Human E2F4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of E2F4/E2F5 (L50/83) pAb in paraffin-embedded human lung carcinoma tissue.)

Related Product Information for anti-E2F4 antibody

E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcriptional regulators including E2F-2, E2F-3, E2F-4, E2F-5 and E2F-6, each of which forms heterodimers with a second protein, DP-1, forming an "active" E2F transcriptional regulatory complex.

NCBI and Uniprot Product Information

• NCBI GI #: 2494229
• NCBI GeneID: 1874
• UniProt Accession #: Q16254; Q15329
• Molecular Weight: ~ 44kDa
• NCBI Official Full Name: Transcription factor E2F4
• NCBI Official Synonym Full Names: E2F transcription factor 4
• NCBI Official Symbol: E2F4
• NCBI Official Synonym Symbols: E2F-4
• NCBI Protein Information: transcription factor E2F4
• UniProt Protein Name: Transcription factor E2F4
• Protein Family: Transcription factor
• UniProt Gene Name: E2F4
• UniProt Synonym Gene Names: E2F-4
• UniProt Entry Name: E2F4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

E2F4: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Belongs to the E2F/DP family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; transcription factor complex

Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription factor activity; transcription factor binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; transcription initiation from RNA polymerase II promoter; organ morphogenesis; transcription, DNA-dependent; blood circulation; transforming growth factor beta receptor signaling pathway; epithelial cell development; gene expression; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; cell volume homeostasis; regulation of cell size; regulation of cell proliferation