Rabbit TFDP1 Polyclonal Antibody | anti-TFDP1 antibody

TFDP1 Polyclonal Antibody

Cat. Num. AAA3010604

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TFDP1; Polyclonal Antibody; TFDP1 Polyclonal Antibody; DP - 1 Transcription Factor; E2F dimerization partner 1; DRTF1-polypeptide 1; DRTF1; DP1; 7027; 189902; Q14186; Dp-1; anti-TFDP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TFDP1 polyclonal antibody detects endogenous levels of TFDP1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 410

• Applicable Applications for anti-TFDP1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human TFDP1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat testis using TFDP1 antibody.)

Related Product Information for anti-TFDP1 antibody

The human retinoblastoma gene product appears to play an important role in the negative egulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 forms heterodimers with a second protein, designated DP-1, forming an "active" E2F transcriptional regulatory complex. Additional members of the E2F family include E2F-2, E2F-3, E2F-4, E2F-5 and DP-2.

NCBI and Uniprot Product Information

• NCBI GI #: 6005900
• NCBI Accession #: NP_009042.1
• NCBI GenBank Nucleotide #: NP_009042.1
• UniProt Accession #: Q14186
• NCBI Official Full Name: transcription factor Dp-1
• UniProt Protein Name: Transcription factor Dp-1
• Protein Family: Transcription factor
• UniProt Gene Name: TFDP1
• UniProt Synonym Gene Names: DP1; DRTF1
• UniProt Entry Name: TFDP1_HUMAN

Uniprot Description

TFDP1: Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1/DP complex appears to mediate both cell proliferation and apoptosis. Belongs to the E2F/DP family.

Protein type: DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: nucleoplasm; transcription factor complex

Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription coactivator activity; transcription factor activity; transcription factor binding

Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; epidermis development; transcription, DNA-dependent; apoptosis; anoikis; regulation of transcription from RNA polymerase II promoter; cell proliferation; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; gene expression; mitotic cell cycle; G1/S transition of mitotic cell cycle