Rabbit Lamin B1 Polyclonal Antibody | anti-B1 antibody

Lamin B1 Polyclonal Antibody

Cat. Num. AAA3000263

• Gene Names: LMNB1; LMN; ADLD; LMN2; LMNB
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Lamin B1; Polyclonal Antibody; Lamin B1 Polyclonal Antibody; Lamin-B1; LMNB1; LMN2; LMNB; anti-B1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Lamin B1 polyclonal antibody detects endogenous levels of Lamin B1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 586

• Applicable Applications for anti-B1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:5000-1:20,000; IHC: 1:100-1:1000; IF: 1:100-1:1000; IP: 1:100-1:1000
• Immunogen: Recombinant full length Human Lamin B1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Lamin B1 polyclonal antibody at 1:20000 dilutionLane1:A549 whole cell lysate (40ug) Lane2:DLD whole cell lysate (40ug) Lane3:The Spleen tissue lysate of Mouse (40ug) Lane4:The Lung tissue lysate of Rat (40ug))

Immunofluorescence (IF)

(Immunofluorescence image of MBS3000263 stained HEK293T cells. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Lamin B1 pAb # MBS3000263 (1:200) at 5ug/ml overnight at +4 degree C. The secondary antibody (Red) was Goat Anti-Rabbit IgG (H+L) Rhodamine (TRITC) used at a 1/2000 dilution for 1h. DAPI was used to stain the cell nuclei (blue).)

Immunoprecipitation (IP)

(Immunoprecipitation of the Lung tissue lysate of Mouse using Lamin B1 pAb (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4). Lane 1 is 30% input. The Western Blot was probed using Lamin B1 pAb # MBS3000263. “up-arrow” (supernatant) ; “down-arrow” (deposition))

Related Product Information for anti-B1 antibody

An important part of the nucleus is formed by nuclear lamina. Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A type lamins and B type lamins. The A type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. The nuclear lamins comprise a unique subclass of the intermediate filament protein family. They share a molecular domain organisation with the other intermediate filament proteins in that they are fibrous molecules that have an aminoterminal globular head, a central rod of alpha helices and a carboxy terminal globular domain. Many biochemical and molecular features of lamins have been studied, but their functions remain still largely undetermined. One of the functions ascribed to the lamina is the maintenance of the structural integrity of the nucleus.

NCBI and Uniprot Product Information

• NCBI GI #: 125953
• NCBI GeneID: 4001
• UniProt Accession #: P20700
• Molecular Weight: ~ 68kDa
• NCBI Official Full Name: Lamin-B1
• NCBI Official Synonym Full Names: lamin B1
• NCBI Official Symbol: LMNB1
• NCBI Official Synonym Symbols: LMN; ADLD; LMN2; LMNB
• NCBI Protein Information: lamin-B1
• UniProt Protein Name: Lamin-B1
• Protein Family: Light-harvesting protein
• UniProt Gene Name: LMNB1
• UniProt Synonym Gene Names: LMN2; LMNB
• UniProt Entry Name: LMNB1_HUMAN

NCBI Description

This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Uniprot Description

Lamin B1: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Nuclear lamins are cleaved by caspases during apoptosis.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 5q23.2

Cellular Component: nucleoplasm; nuclear membrane; membrane; lamin filament; nuclear inner membrane; nuclear envelope

Molecular Function: phospholipase binding; structural molecule activity

Biological Process: apoptosis; cell structure disassembly during apoptosis

Disease: Leukodystrophy, Demyelinating, Adult-onset, Autosomal Dominant