Western Blot (WB)
(Western blot detection of Lamin B1 in C6,3T3,Hela cell lysates using Lamin B1 Rabbit pAb(1:1000 diluted). Predicted band size:66kDa. Observed band size:66kDa.)
Rabbit Lamin B1 Polyclonal Antibody | anti-LMNB1 antibody
Lamin B1 Rabbit pAb
IHC: 1:50
ICC/IF: 1:50
IP: 1:20
Western Blot (WB)
(Western blot detection of Lamin B1 in C6,3T3,Hela cell lysates using Lamin B1 Rabbit pAb(1:1000 diluted). Predicted band size:66kDa. Observed band size:66kDa.)
Western Blot (WB)
(Western blot detection of Lamin B1 in C6,3T3,Hela cell lysates using Lamin B1 Rabbit pAb(1:1000 diluted). Predicted band size:66kDa. Observed band size:66kDa.)
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.MiscellaneousThe structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively.
NCBI and Uniprot Product Information
NCBI Description
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010]
Uniprot Description
Lamin B1: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Nuclear lamins are cleaved by caspases during apoptosis.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 5q23.2
Cellular Component: nucleoplasm; nuclear membrane; membrane; lamin filament; nuclear inner membrane; nuclear envelope
Molecular Function: phospholipase binding; structural molecule activity
Biological Process: apoptosis; cell structure disassembly during apoptosis
Disease: Leukodystrophy, Demyelinating, Adult-onset, Autosomal Dominant
Research Articles on LMNB1
Similar Products
Product Notes
The LMNB1 lmnb1 (Catalog #AAA4752062) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Lamin B1 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Lamin B1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:2000 IHC: 1:50 ICC/IF: 1:50 IP: 1:20. Researchers should empirically determine the suitability of the LMNB1 lmnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lamin B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
