Western Blot (WB)
(Western blot analysis of ADAMTS10 using HuvEc whole cell lysates)
Rabbit anti-Human, Mouse ADAMTS10 Polyclonal Antibody | anti-ADAMTS10 antibody
ADAMTS10 Antibody
Gene Names
ADAMTS10; WMS; WMS1; ADAM-TS10; ADAMTS-10
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ADAMTS10; Polyclonal Antibody; ADAMTS10 Antibody; A disintegrin and metalloproteinase with thrombospondin motifs 10; A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 10; A disintegrin-like and metallopeptidase with thrombospondin type 1 motif; ADAM metallopeptidase with thrombospondin type 1 motif 10; ADAM TS10; ADAM-TS 10; ADAM-TS10; ADAMTS-10; Adamts10; ATS10_HUMAN; EC 3.4.24; WMS; WMS1; zinc metalloendopeptidase; anti-ADAMTS10 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ADAMTS10 antibody detects endogenous levels of total ADAMTS10
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
590
Applicable Applications for anti-ADAMTS10 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human ADAMTS10
Subcellular Location
Secreted > Extracellular Space > Extracellular Matrix.
Tissue Specificity
Widely expressed in adult tissues.
Predicted Cross Reactivity
Pig, Bovine, Rabbit, Dog
Similarity
Pig (100%), Bovine (89%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of ADAMTS10 using HuvEc whole cell lysates)
Western Blot (WB)
(Western blot analysis of ADAMTS10 using HuvEc whole cell lysates)
Related Product Information for anti-ADAMTS10 antibody
Function: Metalloprotease that participate in microfibrils assembly. Microfibrils are extracellular matrix components occurring independently or along with elastin in the formation of elastic tissues.
Subunit Structure: Interacts with FBN1; this interaction promotes microfibrils assembly.
Post-translational Modifications: Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1, 3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).
Similarity: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
Subunit Structure: Interacts with FBN1; this interaction promotes microfibrils assembly.
Post-translational Modifications: Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X2-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1, 3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity).
Similarity: The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 121 kDa
Predicted: 121 kDa
Predicted: 121 kDa
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 10 isoform 2
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif 10
NCBI Official Symbol
ADAMTS10
NCBI Official Synonym Symbols
WMS; WMS1; ADAM-TS10; ADAMTS-10
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 10
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 10
UniProt Gene Name
ADAMTS10
UniProt Synonym Gene Names
ADAM-TS 10; ADAM-TS10; ADAMTS-10
NCBI Description
This gene belongs to the ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin type-1 motifs) family of zinc-dependent proteases. ADAMTS proteases are complex secreted enzymes containing a prometalloprotease domain of the reprolysin type attached to an ancillary domain with a highly conserved structure that includes at least one thrombospondin type 1 repeat. They have been demonstrated to have important roles in connective tissue organization, coagulation, inflammation, arthritis, angiogenesis and cell migration. The product of this gene plays a major role in growth and in skin, lens, and heart development. It is also a candidate gene for autosomal recessive Weill-Marchesani syndrome. [provided by RefSeq, Jul 2008]
Uniprot Description
Metalloprotease that participate in microfibrils assembly. Microfibrils are extracellular matrix components occurring independently or along with elastin in the formation of elastic tissues.
Research Articles on ADAMTS10
Similar Products
Product Notes
The ADAMTS10 adamts10 (Catalog #AAA9609486) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ADAMTS10 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ADAMTS10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the ADAMTS10 adamts10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADAMTS10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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