Rabbit PIK3R4 Polyclonal Antibody | anti-PIK3R4 antibody

PIK3R4 Polyclonal Antibody

Cat. Num. AAA3000255

• Gene Names: PIK3R4; p150; VPS15
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PIK3R4; Polyclonal Antibody; PIK3R4 Polyclonal Antibody; Phosphoinositide 3-kinase regulatory subunit 4; PI3-kinase regulatory subunit 4; PI3-kinase p150 subunit; Phosphoinositide 3-kinase adaptor protein; VPS15; anti-PIK3R4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PIK3R4 polyclonal antibody detects endogenous levels of PIK3R4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1358

• Applicable Applications for anti-PIK3R4 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding Human PIK3R4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PIK3R4 polyclonal antibody at 1:500 dilutionLane1:The Testis tissue lysate of Mouse (40ug) Lane2:The Testis tissue lysate of Rat (40ug) Lane3:HCC827 whole cell lysate (40ug) Lane4:L02 whole cell lysate (40ug) Lane5:SK-OVCAR3 whole cell lysate (40ug))

Testing Data

Related Product Information for anti-PIK3R4 antibody

The serine/threonine PI3 kinase regulatory subunit 4 (PIK3R4, Vps15) is the mammalian homologue of the yeast vacuolar protein sorting 15. PIK3R4 regulates the kinase activity of PI3K class III and anchors the kinase to cellular membranes through myristoylation. Recruitment of PI3K class III to the site of early endosome fusion and docking is directly mediated by PIK3R4 binding to the small GTPase Rab5 through its HEAT and WD-40 domains. The PIK3R4/PI3K class III plays a role in late endosome function through PIK3R4 binding to the Rab7 GTPase. In addition to its role in trafficking, the PIK3R4/PI3K class III complex interacts with beclin-1 to play a role during several stages of autophagy. Autophagosome formation is stimulated when Atg14 complexes with PIK3R4, PI3K class III, and beclin-1. The UVRAG protein competes with Atg14 for beclin-1 binding, forming a mutually exclusive complex with PIK3R4, PI3K class III, and beclin-1 that regulates autophagosome maturation. Autophagosome maturation is impaired in the presence of the beclin-1-binding protein Rubicon. Co-expression of PIK3R4 is required for PI3K class III activation and regulation by both beclin-1/UVRAG and by nutrients. Overexpression of PIK3R4 protein has been associated with decreased survival in patients with ovarian tumors, while mutations of the corresponding PIK3R4 gene are associated with metastatic melanoma, suggesting that PIK3R4 functions in cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 23943912
• NCBI GeneID: 30849
• NCBI Accession #: NP_055417
• NCBI GenBank Nucleotide #: NP_055417.1
• UniProt Accession #: Q99570
• Molecular Weight: ~ 153kDa
• NCBI Official Full Name: phosphoinositide 3-kinase regulatory subunit 4
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase regulatory subunit 4
• NCBI Official Symbol: PIK3R4
• NCBI Official Synonym Symbols: p150; VPS15
• NCBI Protein Information: phosphoinositide 3-kinase regulatory subunit 4
• UniProt Protein Name: Phosphoinositide 3-kinase regulatory subunit 4
• Protein Family: Phosphoinositide 3-kinase regulatory
• UniProt Gene Name: PIK3R4
• UniProt Synonym Gene Names: PI3-kinase regulatory subunit 4
• UniProt Entry Name: PI3R4_HUMAN

Uniprot Description

PIK3R4: Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Kinase, lipid; Protein kinase, Other; Other group; VPS15 family

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: membrane; late endosome; nuclear pore; axoneme; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: macroautophagy; phospholipid metabolic process; late endosome to vacuole transport; toll-like receptor signaling pathway; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; innate immune response; protein targeting to vacuole; toll-like receptor 9 signaling pathway; peroxisome degradation; protein amino acid phosphorylation