Rabbit PIK3R4 Polyclonal Antibody | anti-PIK3R4 antibody

PIK3R4 Antibody

Cat. Num. AAA153430

• Gene Names: PIK3R4; p150; VPS15
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: PIK3R4 antibody is affinity chromatography purified via peptide column.
• Synonyms: PIK3R4; Polyclonal Antibody; PIK3R4 Antibody; Phosphoinositide-3-kinase regulatory subunit 4; p150; Phosphoinositide 3-kinase adaptor protein; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; VPS15; phosphoinositide 3-kinase regulatory subunit 4; anti-PIK3R4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PIK3R4 antibody is human, mouse and rat reactive.
• Purity/Purification: PIK3R4 antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1358

• Applicable Applications for anti-PIK3R4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: PIK3R4 antibody can be used for detection of PIK3R4 by Western blot at 1 - 2 mug/ml. Antibody can also be used for Immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: PIK3R4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PIK3R4.
• Buffer: PIK3R4 antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: PIK3R4 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of PIK3R4 in human tonsil tissue lysate with PIK3R4 antibody at (A) 1 and (B) 2 μg/ml.)

Immunohistochemistry (IHC)

(Immunohistochemistry of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 5 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of PIK3R4 in human tonsil tissue with PIK3R4 antibody at 20 μg/ml.)

Related Product Information for anti-PIK3R4 antibody

PIK3R4 (phosphoinositide-3-kinase regulatory subunit 4, also known as VPS15) is a 150kD adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway and autophagy (1,2). PIK3R4 is ubiquitously expressed and contains 7 WD repeats, 3 HEAT repeats and 1 protein kinase domain (1). PIK3R4 is crucial for VPS34 function and is necessary for autophagosome formation in multicellular animals and reveal an important role of VPS15 in cellular protection against ubiquitin-positive protein aggregates (3-5).

NCBI and Uniprot Product Information

• NCBI GI #: 23943912
• NCBI GeneID: 30849
• NCBI Accession #: NP_055417
• NCBI GenBank Nucleotide #: NM_014602.2
• UniProt Accession #: Q99570; Q2TBF4
• Molecular Weight: Predicted: 149 kDa; ; Observed: 140 kDa
• NCBI Official Full Name: phosphoinositide 3-kinase regulatory subunit 4
• NCBI Official Synonym Full Names: phosphoinositide-3-kinase, regulatory subunit 4
• NCBI Official Symbol: PIK3R4
• NCBI Official Synonym Symbols: p150; VPS15
• NCBI Protein Information: phosphoinositide 3-kinase regulatory subunit 4; PI3-kinase p150 subunit; PI3-kinase regulatory subunit 4; phosphoinositide 3-kinase adaptor protein; phosphatidylinositol 3-kinase-associated p150; phosphoinositide-3-kinase, regulatory subunit 4, p150
• UniProt Protein Name: Phosphoinositide 3-kinase regulatory subunit 4
• Protein Family: Phosphoinositide 3-kinase regulatory
• UniProt Gene Name: PIK3R4
• UniProt Synonym Gene Names: PI3-kinase regulatory subunit 4
• UniProt Entry Name: PI3R4_HUMAN

Uniprot Description

PIK3R4: Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Kinase, lipid; Protein kinase, Other; Other group; VPS15 family

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: membrane; late endosome; nuclear pore; axoneme; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: macroautophagy; phospholipid metabolic process; late endosome to vacuole transport; toll-like receptor signaling pathway; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; innate immune response; protein targeting to vacuole; toll-like receptor 9 signaling pathway; peroxisome degradation; protein amino acid phosphorylation