Bovine Tissue Inhibitor of Metalloproteinases 1 ELISA Kit | TIMP-1 elisa kit
Bovine Tissue Inhibitor of Metalloproteinases 1 ELISA Kit
Reactivity
Bovine
Synonyms
Tissue Inhibitor of Metalloproteinases 1; Bovine Tissue Inhibitor of Metalloproteinases 1 ELISA Kit; TIMP-1 elisa kit
Reactivity
Bovine
Specificity
No significant cross-reactivity or interference between Bovine TIMP-1 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type
Sandwich
Detection Range
6.25ng/ml-200ng/ml.
Sensitivity
1.0ng/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated TIMP-1 concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine TIMP-1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TIMP-1 and TIMP-1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TIMP-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Related Product Information for TIMP-1 elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated TIMP-1 concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine TIMP-1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add TIMP-1 and TIMP-1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of TIMP-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23,032 Da
NCBI Official Full Name
TIMP-1
NCBI Official Synonym Full Names
TIMP metallopeptidase inhibitor 1
NCBI Official Symbol
TIMP1
NCBI Protein Information
metalloproteinase inhibitor 1; EG-1; TIMP-1; embryogenin-1; tissue inhibitor of metalloproteinases 1; tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)
UniProt Protein Name
Metalloproteinase inhibitor 1
UniProt Gene Name
TIMP1
UniProt Synonym Gene Names
EG-1; TIMP-1
UniProt Entry Name
TIMP1_BOVIN
Uniprot Description
Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
Subcellular location: Secreted.
Post-translational modification: The activity of TIMP1 is dependent on the presence of disulfide bonds.
Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family. [View classification]Contains 1 NTR domain.
Research Articles on TIMP-1
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Product Notes
The Bovine TIMP-1 timp1 (Catalog #AAA029805) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA029805 ELISA Kit recognizes Bovine TIMP-1. It is sometimes possible for the material contained within the vial of "Tissue Inhibitor of Metalloproteinases 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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