Typical Testing Data/Standard Curve (for reference only)
Goat Tissue Inhibitor of Metalloproteinases 1 ELISA Kit | TIMP-1 elisa kit
Goat Tissue Inhibitor of Metalloproteinases 1 ELISA Kit
Reactivity
Goat
Synonyms
Tissue Inhibitor of Metalloproteinases 1; Goat Tissue Inhibitor of Metalloproteinases 1 ELISA Kit; TIMP-1 elisa kit
Reactivity
Goat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Intended Uses
This TIMP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat TIMP-1. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TIMP-1 elisa kit
Principle of the assay: TIMP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TIMP-1 antibody and an TIMP-1-HRP conjugate. The assay sample and buffer are incubated together with TIMP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TIMP-1 concentration since TIMP-1 from samples and TIMP-1-HRP conjugate compete for the anti-TIMP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by TIMP-1 from the sample, fewer sites are left to bind TIMP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TIMP-1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for TIMP-1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23,032 Da
NCBI Official Full Name
TIMP-1
NCBI Official Synonym Full Names
TIMP metallopeptidase inhibitor 1<
NCBI Official Symbol
TIMP1
NCBI Protein Information
metalloproteinase inhibitor 1; EG-1; TIMP-1; embryogenin-1; tissue inhibitor of metalloproteinases 1; tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)
UniProt Protein Name
Metalloproteinase inhibitor 1
UniProt Gene Name
TIMP1
UniProt Synonym Gene Names
EG-1; TIMP-1
UniProt Entry Name
TIMP1_BOVIN
Uniprot Description
Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
Subcellular location: Secreted.
Post-translational modification: The activity of TIMP1 is dependent on the presence of disulfide bonds.
Sequence similarities: Belongs to the protease inhibitor I35 (TIMP) family. [View classification]Contains 1 NTR domain.
Research Articles on TIMP-1
Similar Products
Product Notes
The Goat TIMP-1 timp1 (Catalog #AAA737301) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA737301 ELISA Kit recognizes Goat TIMP-1. It is sometimes possible for the material contained within the vial of "Tissue Inhibitor of Metalloproteinases 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
