NCOR1 / Nuclear Receptor Corepressor 1 Recombinant Protein | NCOR1 recombinant protein

Human NCOR1 / Nuclear Receptor Corepressor 1 Recombinant Protein

• Gene Names: NCOR1; N-CoR; TRAC1; N-CoR1; hN-CoR; PPP1R109
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: NCOR1 / Nuclear Receptor Corepressor 1; Human NCOR1 / Nuclear Receptor Corepressor 1 Recombinant Protein; Nuclear receptor corepressor 1; N-CoR; KIAA1047; NCOR1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 914

• Applicable Applications for NCOR1 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Forms a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid receptor (TR) and the retinoid acid receptor (RAR) in the absence of ligand. Interacts directly with RARA; the interaction is facilitated with RARA trimethylation. Component of the N-Cor repressor complex, at least composed of CBFA2T3, HEXIM1, NCOR1, NCOR2, HDAC3, TBL1X, TBL1XR1, CORO2A and GPS2. Interacts with ZBTB33; the interaction serves to recruit the N-CoR complex to promoter regions containing methylated CpG dinucleotides. Interacts with TRIM28 and KDM3A. Interacts (via the RD1 domain) with BAZ1A (via its N-terminal); the interaction corepresses a number of NCOR1-regulated genes. Interacts with BCL6, C1D, DACH1, HEXIM1, HDAC7, RORA, RORC, SAP30, SIAH2, SIN3A and SIN3B. May interact with DEAF1. Interacts with RXRA.
• Entry Function: Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. Participates in the transcriptional repressor activity produced by BCL6.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 298919179
• NCBI GeneID: 9611
• NCBI Accession #: NP_001177367.1
• NCBI GenBank Nucleotide #: NP_001177367.1
• UniProt Accession #: O75376
• NCBI Official Full Name: nuclear receptor corepressor 1 isoform 2
• NCBI Official Synonym Full Names: nuclear receptor corepressor 1
• NCBI Official Symbol: NCOR1
• NCBI Official Synonym Symbols: N-CoR; TRAC1; N-CoR1; hN-CoR; PPP1R109
• NCBI Protein Information: nuclear receptor corepressor 1
• UniProt Protein Name: Nuclear receptor corepressor 1
• Protein Family: Nuclear receptor corepressor
• UniProt Gene Name: NCOR1
• UniProt Synonym Gene Names: KIAA1047; N-CoR; N-CoR1
• UniProt Entry Name: NCOR1_HUMAN

NCBI Description

This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.[provided by RefSeq, Jun 2010]

Uniprot Description

N-CoR1: is a protein that regulates the activity of some transcription factors, including nuclear receptors, by promoting histone deacetylation and chromatin condensation, thereby altering the accessibility of various genes to the transcriptional machinery. Is known to play a role genetic programming including that for myogenesis. Is a component of a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid and the retinoic acid receptors in the absence of ligand. Interacts with the catalytic domain of HDAC9. Increased expression correlates with the loss of vitamin D responsiveness in aggressive androgen-independent prostate cancer cells. Its gene tends to be overexpressed in multiple-myeloma cell lines.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; Sin3 complex; membrane; spindle microtubule; nuclear chromatin; histone deacetylase complex; cytoplasm; nucleus

Molecular Function: retinoid X receptor binding; protein binding; histone deacetylase regulator activity; sequence-specific DNA binding; histone deacetylase binding; thyroid hormone receptor binding; chromatin binding; transcription factor activity; transcription corepressor activity; nuclear hormone receptor binding

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; negative regulation of JNK cascade; CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; Notch signaling pathway; transcription, DNA-dependent; regulation of multicellular organism growth; chromatin modification; thalamus development; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; spindle assembly; positive regulation of histone deacetylation; cholesterol homeostasis; transforming growth factor beta receptor signaling pathway; gene expression; circadian regulation of gene expression; negative regulation of phosphoinositide 3-kinase cascade