Bovine Ubiquitin-like protein ISG15 ELISA Kit | ISG15 elisa kit

Bovine Ubiquitin-like protein ISG15 ELISA Kit

Cat. Num. AAA289099

• Reactivity: Bovine
• Synonyms: Ubiquitin-like protein ISG15; Bovine Ubiquitin-like protein ISG15 ELISA Kit; Interferon-stimulated gene product 17; Ubiquitin cross-reactive protein; BoUCRP; ISG15; G1P2; ISG17; UCRP; ISG15 elisa kit

• Reactivity: Bovine
• Specificity: This assay recognizes recombinant and natural bovine Ubiquitin-like protein ISG15. No significant cross-reactivity or interference was observed.
• Sequence Length: 154

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids
• Detection Range: 31.2 pg/mL -2000 pg/mL.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ISG15 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of bovine Ubiquitin-like protein ISG15 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin-like protein ISG15. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ubiquitin-like protein ISG15 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin-like protein ISG15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Ubiquitin-like protein ISG15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27805955
• NCBI GeneID: 281871
• NCBI Accession #: NP_776791.1
• NCBI GenBank Nucleotide #: NM_174366.1
• UniProt Accession #: O02741; Q3T0N6
• Molecular Weight: 17,310 Da
• NCBI Official Full Name: ubiquitin-like protein ISG15
• NCBI Official Symbol: ISG15
• NCBI Protein Information: ubiquitin-like protein ISG15
• UniProt Protein Name: Ubiquitin-like protein ISG15
• Protein Family: Ubiquitin-like protein
• UniProt Gene Name: ISG15
• UniProt Synonym Gene Names: G1P2; ISG17; UCRP; BoUCRP
• UniProt Entry Name: ISG15_BOVIN

Uniprot Description

Ubiquitin-like protein which plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. ISGylation involves a cascade of enzymatic reactions involving E1, E2, and E3 enzymes which catalyze the conjugation of ISG15 to a lysine residue in the target protein. Exhibits antiviral activity towards both DNA and RNA viruses. The secreted form of ISG15 can: induce natural killer cell proliferation, augment lymphokine-activated-killer (LAK) activity, induce dendritic cell maturation, act as a chemotactic factor for neutrophils and act as a IFN-gamma-inducing cytokine playing an essential role in antimycobacterial immunity (). In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.