Mouse ISG15 ubiquitin-like modifier ELISA Kit | ISG15 elisa kit

Mouse Ubiquitin-like protein ISG15, ISG15 ELISA Kit

Cat. Num. AAA938670

• Gene Names: Isg15; G1p2; IP17; Irfp; UCRP; IGI15
• Reactivity: Mouse
• Synonyms: ISG15 ubiquitin-like modifier; Mouse Ubiquitin-like protein ISG15; ISG15 ELISA Kit; Mouse Ubiquitin-like protein ISG15 (ISG15) ELISA kit; G1P2; IFI15; UCRP; interferon; alpha-inducible protein (clone IFI-15K) ; interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein; 15 kDa; ubiquitin cross-reactive protein; ISG15 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse ISG15. No significant cross-reactivity or interference between mouse ISG15 and analogues was observed.
• Sequence Length: 161

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312 ng/ml-20 ng/ml
• Sensitivity: < 0.078 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ISG15 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ISG15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ISG15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ISG15 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ISG15 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 226874851
• NCBI GeneID: 100038882
• NCBI Accession #: NP_056598.2
• NCBI GenBank Nucleotide #: NM_015783.3
• UniProt Accession #: Q64339; Q8K0H3
• Molecular Weight: 17,898 Da
• NCBI Official Full Name: ubiquitin-like protein ISG15
• NCBI Official Synonym Full Names: ISG15 ubiquitin-like modifier
• NCBI Official Symbol: Isg15
• NCBI Official Synonym Symbols: G1p2; IP17; Irfp; UCRP; IGI15
• NCBI Protein Information: ubiquitin-like protein ISG15; interferon-stimulated protein 15; ubiquitin cross-reactive protein; interferon-induced 15 kDa protein; interferon-induced 15-KDa protein; interferon-induced 17 kDa protein; interferon, alpha-inducible protein; interferon-stimulated protein (15 kDa)
• UniProt Protein Name: Ubiquitin-like protein ISG15
• Protein Family: Ubiquitin-like protein
• UniProt Gene Name: Isg15
• UniProt Synonym Gene Names: G1p2; Ucrp; IP17
• UniProt Entry Name: ISG15_MOUSE

Uniprot Description

ISG15: Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation. By type I interferons. Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.

Protein type: Ubiquitin-like modifier

Cellular Component: cytoplasm; extracellular region

Molecular Function: protein tag; protein binding

Biological Process: ISG15-protein conjugation; modification-dependent protein catabolic process; negative regulation of viral genome replication; regulation of interferon-gamma production; positive regulation of erythrocyte differentiation; defense response to bacterium; negative regulation of protein ubiquitination; defense response to virus