Mouse R-spondin-1 ELISA Kit | Rspo1 elisa kit

Mouse R-spondin-1 ELISA Kit

Cat. Num. AAA2885665

• Gene Names: Rspo1; Rspondin; R-spondin
• Reactivity: Mouse
• Synonyms: R-spondin-1; Mouse R-spondin-1 ELISA Kit; Cysteine-rich and single thrombospondin domain-containing protein 3; Cristin-3; mCristin-3; Roof plate-specific spondin-1; Rspo1; Rspo1 elisa kit

• Reactivity: Mouse
• Specificity: Recombinant and natural Mouse R-spondin-1
• Sequence Length: 265

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Rspo1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 227452351
• NCBI GeneID: 192199
• NCBI Accession #: NP_619624.2
• NCBI GenBank Nucleotide #: NM_138683.2
• UniProt Accession #: Q9Z132; Q3V1S3
• Molecular Weight: 29,332 Da
• NCBI Official Full Name: R-spondin-1
• NCBI Official Synonym Full Names: R-spondin 1
• NCBI Official Symbol: Rspo1
• NCBI Official Synonym Symbols: Rspondin; R-spondin
• NCBI Protein Information: R-spondin-1
• UniProt Protein Name: R-spondin-1
• Protein Family: R-spondin
• UniProt Gene Name: Rspo1
• UniProt Synonym Gene Names: Cristin-3; mCristin-3
• UniProt Entry Name: RSPO1_MOUSE

Uniprot Description

R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cell development/differentiation; Cell adhesion

Cellular Component: extracellular region; extracellular space; nucleus

Molecular Function: G-protein-coupled receptor binding; heparin binding; protein binding; receptor binding

Biological Process: male meiosis; positive regulation of protein amino acid phosphorylation; positive regulation of Wnt receptor signaling pathway; regulation of gene expression; regulation of receptor internalization; response to stimulus; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin