Typical Testing Data/Standard Curve (for reference only)
Human R-spondin-1, RSPO1 ELISA Kit | RSPO1 elisa kit
Human R-spondin-1, RSPO1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human RSPO1 antibody. RSPO1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human RSPO1 Antibody is added and binds to RSPO1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated RSPO1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human RSPO1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
Uniprot Description
R-spondin-1: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Defects in RSPO1 are the cause of palmoplantar keratoderma with squamous cell carcinoma of skin and sex reversal (PKKSCC). This recessive syndrome is characterized by XX (female to male) SRY-independent sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Cell development/differentiation; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4|4 D2.2
Cellular Component: extracellular space; nucleus
Molecular Function: G-protein-coupled receptor binding; heparin binding; protein binding; receptor binding
Biological Process: male meiosis; positive regulation of protein amino acid phosphorylation; positive regulation of Wnt receptor signaling pathway; regulation of gene expression; regulation of receptor internalization; Wnt receptor signaling pathway through beta-catenin
Research Articles on RSPO1
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Product Notes
The Human RSPO1 rspo1 (Catalog #AAA1606963) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1606963 ELISA Kit recognizes Human RSPO1. It is sometimes possible for the material contained within the vial of "R-spondin-1, RSPO1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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