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Typical Testing Data/Standard Curve (for reference only)

Rat 5-hydroxytryptamine (serotonin) receptor 2A ELISA Kit | HTR2A elisa kit

Rat Serotonin Receptor 2A/5-hydroxytryptamine Receptor 2A, 5-HT-2A ELISA Kit

Gene Names
Htr2a; 5Ht-2; 5-HT2A
Reactivity
Rat
Synonyms
5-hydroxytryptamine (serotonin) receptor 2A; Rat Serotonin Receptor 2A/5-hydroxytryptamine Receptor 2A; 5-HT-2A ELISA Kit; Rat Serotonin Receptor 2A/5-hydroxytryptamine Receptor 2A (5-HT-2A) ELISA Kit; RP11-147L20.2; 5-HT2A; HTR2; 5-HT2 receptor; 5-hydroxytryptamine receptor 2A; serotonin 5-HT-2A receptor; HTR2A elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat 5-HT-2A. No significant cross-reactivity or interference between rat 5-HT-2A and analogues was observed.
Sequence Length
471
Samples
serum, plasma, tissue homogenates, cell ysates
Assay Type
Quantitative Sandwich
Detection Range
31.25 pg/ml -2000 pg/ml
Sensitivity
<7.8 pg/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HTR2A elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for 5-HT-2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any 5-HT-2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 5-HT-2A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 5-HT-2A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52,850 Da
NCBI Official Full Name
5-hydroxytryptamine receptor 2A
NCBI Official Synonym Full Names
5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled
NCBI Official Symbol
Htr2a
NCBI Official Synonym Symbols
5Ht-2; 5-HT2A
NCBI Protein Information
5-hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2A; serotonin receptor 2A; serotonin 5HT-2 receptor
UniProt Protein Name
5-hydroxytryptamine receptor 2A
UniProt Gene Name
Htr2a
UniProt Synonym Gene Names
Htr2; 5-HT-2; 5-HT-2A
UniProt Entry Name
5HT2A_RAT

NCBI Description

a receptor for 5-hydroxytryptamine (serotonin); activates phospholipase C signaling pathway; involved in promoting cellular transformation [RGD, Feb 2006]

Uniprot Description

Function: G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca2+ ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.2 PublicationsManual assertion based on experiment in:Ref.1

Research Articles on HTR2A

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Product Notes

The Rat HTR2A htr2a (Catalog #AAA705328) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA705328 ELISA Kit recognizes Rat HTR2A. It is sometimes possible for the material contained within the vial of "5-hydroxytryptamine (serotonin) receptor 2A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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