Typical Testing Data/Standard Curve (for reference only)
Mouse Tryptophanyl-tRNA synthetase, cytoplasmic ELISA Kit | Wars elisa kit
Mouse Tryptophanyl-tRNA synthetase, cytoplasmic ELISA Kit
Gene Names
Wars; WRS; TrpRS
Reactivity
Mouse
Synonyms
Tryptophanyl-tRNA synthetase; cytoplasmic; Mouse Tryptophanyl-tRNA synthetase; cytoplasmic ELISA Kit; Tryptophan--tRNA ligase; TrpRS; Wars; Wrs; 6.1.1.2; Wars elisa kit
Reactivity
Mouse
Specificity
This assay recognizes recombinant and natural mouseTryptophanyl-tRNAsynthetase, cytoplasmic. No significant cross-reactivity or interference was observed.
Sequence Length
475
Assay Type
Sandwich
Detection Range
0.156-10 ng/mL
Sensitivity
0.088 ng/mL
Intra-Assay CV
<=6.2%
Inter-Assay CV
<=8.5%
Recovery
103%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Wars elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination ofmouseTryptophanyl-tRNAsynthetase, cytoplasmicconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptophanyl-tRNAsynthetase, cytoplasmic. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forTryptophanyl-tRNAsynthetase, cytoplasmic and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tryptophanyl-tRNAsynthetase, cytoplasmic, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Tryptophanyl-tRNAsynthetase, cytoplasmic in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptophanyl-tRNAsynthetase, cytoplasmic. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forTryptophanyl-tRNAsynthetase, cytoplasmic and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tryptophanyl-tRNAsynthetase, cytoplasmic, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Tryptophanyl-tRNAsynthetase, cytoplasmic in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,641 Da
NCBI Official Full Name
tryptophan--tRNA ligase, cytoplasmic isoform 2
NCBI Official Synonym Full Names
tryptophanyl-tRNA synthetase
NCBI Official Symbol
Wars
NCBI Official Synonym Symbols
WRS; TrpRS
NCBI Protein Information
tryptophan--tRNA ligase, cytoplasmic
UniProt Protein Name
Tryptophan--tRNA ligase, cytoplasmic
Protein Family
UniProt Gene Name
Wars
UniProt Synonym Gene Names
Wrs; TrpRS
UniProt Entry Name
SYWC_MOUSE
Uniprot Description
WARS: Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Belongs to the class-I aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - tryptophan; EC 6.1.1.2; Ligase; Translation
Cellular Component: cytoplasm; nucleus
Molecular Function: tryptophan-tRNA ligase activity
Biological Process: regulation of angiogenesis; tryptophanyl-tRNA aminoacylation
Research Articles on Wars
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Product Notes
The Mouse Wars wars (Catalog #AAA2884685) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2884685 ELISA Kit recognizes Mouse Wars. It is sometimes possible for the material contained within the vial of "Tryptophanyl-tRNA synthetase, cytoplasmic, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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